Rafet Mete1, Mustafa Oran2, Birol Topcu3, Meltem Oznur4, Erdogan Selcuk Seber5, Asuman Gedikbasi6, Tarkan Yetisyigit7. 1. Department of Gastroenterology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey rafetmete@yahoo.com. 2. Department of Internal Medicine, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey. 3. Department of Biostatistics, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey. 4. Department of Pathology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey. 5. Medical Oncology Clinic, Tekirdag State Hospital, Tekirdag, Turkey. 6. Biochemistry Department, Sadi Konuk Research and Training Hospital, İstanbul, Turkey. 7. Department of Medical Oncology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
Abstract
BACKGROUND/AIM: Doxorubicin (DOX) is a widely used and potent chemotherapeutic agent. However, serious dose-limiting toxicity through generation of free oxygen radicals is a commonly encountered clinical problem. The aim of the current study was to assess the protective role of onion (Allium cepa) extract (ACE) against DOX-induced hepatotoxicity in rats. METHOD: A total of 24 rats were randomly divided into 3 equal experimental groups: (1) DOX; (2) ACE + DOX; and (3) control groups. ACE was given orally as 1 mL of fresh ACE juice for 14 consecutive days followed by DOX injection. DOX was injected intraperitoneally in a single dose of 30 mg/kg body weight to induce hepatotoxicity, and the rats were killed after 48 h from injection. Control group was given saline only. RESULTS: In the ACE pretreated group (ACE + DOX), serum aspartate transaminase, alanine transaminase, and tissue malondialdehyde and glutathione levels were significantly lower, while superoxide dismutase and glutathione peroxidase were higher compared with the DOX group. The histopathological examination of liver specimens revealed parenchymal necrosis, proliferation of biliary duct in DOX group; while ACE pretreatment provided marked reduction in these changes. CONCLUSION: Our study indicates that pretreatment with ACE protects against DOX-induced hepatotoxicity due to the antioxidant properties of ACE. Further studies on efficacy of antioxidant treatment by ACE in DOX-mediated toxicity and underlying mechanisms would provide a better explanation.
BACKGROUND/AIM: Doxorubicin (DOX) is a widely used and potent chemotherapeutic agent. However, serious dose-limiting toxicity through generation of free oxygen radicals is a commonly encountered clinical problem. The aim of the current study was to assess the protective role of onion (Allium cepa) extract (ACE) against DOX-induced hepatotoxicity in rats. METHOD: A total of 24 rats were randomly divided into 3 equal experimental groups: (1) DOX; (2) ACE + DOX; and (3) control groups. ACE was given orally as 1 mL of fresh ACE juice for 14 consecutive days followed by DOX injection. DOX was injected intraperitoneally in a single dose of 30 mg/kg body weight to induce hepatotoxicity, and the rats were killed after 48 h from injection. Control group was given saline only. RESULTS: In the ACE pretreated group (ACE + DOX), serum aspartate transaminase, alanine transaminase, and tissue malondialdehyde and glutathione levels were significantly lower, while superoxide dismutase and glutathione peroxidase were higher compared with the DOX group. The histopathological examination of liver specimens revealed parenchymal necrosis, proliferation of biliary duct in DOX group; while ACE pretreatment provided marked reduction in these changes. CONCLUSION: Our study indicates that pretreatment with ACE protects against DOX-induced hepatotoxicity due to the antioxidant properties of ACE. Further studies on efficacy of antioxidant treatment by ACE in DOX-mediated toxicity and underlying mechanisms would provide a better explanation.
Authors: Yoon Hee Cho; Joong Won Lee; Hae Dong Woo; Sunyeong Lee; Yang Jee Kim; Younghyun Lee; Sangah Shin; Hyojee Joung; Hai Won Chung Journal: Int J Environ Res Public Health Date: 2016-02-19 Impact factor: 3.390