Imran A Khan1, Parveen Jahan2, Qurratulain Hasan3, Pragna Rao4. 1. Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Genetics, Vasavi Medical and Research Centre, India Department of Genetics and Biotechnology, Osmania University, India. 2. Department of Genetics and Biotechnology, Osmania University, India. 3. Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Genetics, Vasavi Medical and Research Centre, India. 4. Department of Genetics and Molecular Medicine, Kamineni Hospitals, India Department of Biochemistry, Kamineni Hospitals, India Department of Biochemistry, Kasturba Medical College, Manipal University, India drpragnarao@gmail.com.
Abstract
INTRODUCTION: Gestational diabetes mellitus (GDM) is defined as glucose intolerance first recognized during pregnancy. Insertion/deletion (I/D) polymorphism of a 287 bp Alu repetitive sequence in intron 16 of the angiotensin-converting enzyme (ACE) gene has been widely investigated in Asian Indian populations with different ethnic origins. The present study examined possible association between I/D polymorphism of the ACE gene and GDM in Asian Indian pregnant women. METHODS: A total of 200 pregnant women (100 GDM and 100 non-GDM) were recruited in this study and I/D polymorphism of a 287 bp Alu1 element inside intron 16 of the ACE gene was examined by polymerase chain reaction (PCR)-based gel electrophoresis. RESULT: The distribution of the variants like II, ID, and DD genotypes of ACE gene showed differences between normal GDM versus non-GDM subjects, and the frequency of the ID+ DD Vs II genotype was significant (p=0.0002) in the GDM group. CONCLUSION: ACE gene polymorphism was associated with GDM in Asian Indian pregnant women.
INTRODUCTION:Gestational diabetes mellitus (GDM) is defined as glucose intolerance first recognized during pregnancy. Insertion/deletion (I/D) polymorphism of a 287 bp Alu repetitive sequence in intron 16 of the angiotensin-converting enzyme (ACE) gene has been widely investigated in Asian Indian populations with different ethnic origins. The present study examined possible association between I/D polymorphism of the ACE gene and GDM in Asian Indian pregnant women. METHODS: A total of 200 pregnant women (100 GDM and 100 non-GDM) were recruited in this study and I/D polymorphism of a 287 bp Alu1 element inside intron 16 of the ACE gene was examined by polymerase chain reaction (PCR)-based gel electrophoresis. RESULT: The distribution of the variants like II, ID, and DD genotypes of ACE gene showed differences between normal GDM versus non-GDM subjects, and the frequency of the ID+ DD Vs II genotype was significant (p=0.0002) in the GDM group. CONCLUSION:ACE gene polymorphism was associated with GDM in Asian Indian pregnant women.
Authors: Neda M Bogari; Ashwag Aljohani; Anas Dannoun; Osama Elkhateeb; Masimo Porqueddu; Amr A Amin; Dema N Bogari; Mohiuddin M Taher; Faruk Buba; Reem M Allam; Mustafa N Bogari; Francesco Alamanni Journal: Saudi J Biol Sci Date: 2020-06-24 Impact factor: 4.219