Literature DB >> 24186483

A method to sharply delimit a yeast nuclear gene in a cloned DNA fragment.

G Faye1, M Simon.   

Abstract

We have developped a procedure to delimit the boundaries of a cloned gene carried on a DNA fragment as large as 4 to 5 kilobases. The method consists in the following. Two series of limit digest products generated with a tetranucleotide recognition sequence endonuclease and originating from either of the two ends of this DNA segment are tested for their complementing capacity by yeast transformation. The gene is then delimited by the overlap of the two shortest complementing fragments.

Entities:  

Year:  1982        PMID： 24186483     DOI： 10.1007/BF00435216

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  15 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A simple method for DNA restriction site mapping.

Authors:  H O Smith; M L Birnstiel
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

3.  Construction and characterization of three yeast-Escherichia coli shuttle vectors designed for rapid subcloning of yeast genes on small DNA fragments.

Authors:  J Ferguson; J C Groppe; S I Reed
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

4.  5'-Labeling and poly(dA) tailing.

Authors:  P G Boseley; T Moss; M L Birnstiel
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  Plasmid-linked ampicillin resistance in haempohilus influenza type b.

Authors:  L P Elwell; J De Graaff; D Seibert; S Falkow
Journal:  Infect Immun       Date:  1975-08       Impact factor: 3.441

6.  Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

Authors:  K A Nasmyth; S I Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

7.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Transformation of yeast by a replicating hybrid plasmid.

Authors:  J D Beggs
Journal:  Nature       Date:  1978-09-14       Impact factor: 49.962

9.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  Preliminary characterization of the transcriptional and translational products of the Saccharomyces cerevisiae cell division cycle gene CDC28.

Authors:  S I Reed; J Ferguson; J C Groppe
Journal:  Mol Cell Biol       Date:  1982-04       Impact factor: 4.272
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  1 in total

1.  Primary structure of a gene for subunit V of the cytochrome c oxidase from Saccharomyces cerevisiae.

Authors:  B Séraphin; M Simon; G Faye
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886
  1 in total

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