Literature DB >> 24186477

Minicircular DNA having sequence homologies with chloroplast DNA in a bleached mutant of Euglena gracilis.

P Heizmann1, P Ravel-Chapuis, V Nigon.   

Abstract

Chloroplast DNA was isolated from total cellular DNA of a bleached mutant of Euglena gracilis (Y3BUD) by enrichment of the light component (p = 1.686) by repeated CsCl equilibrium centrifugations. Electron microscope visualization of this DNA showed minicircular DNA molecules in addition to large circular molecules (42 pm) identical to wild type chloroplast DNA. They were heterogenous in size and their contour lengths ranged from 0.8 to 8.5 μm. Fractionnation by agarose gel electrophoresis gave several discrete bands. Some of them hybridized with pure chloroplast DNA and with several cloned chloroplast DNA fragments, particularly to ribosomal fragments, while others did not show homology with chloroplast DNA being probably of extrachloroplatic origin.

Entities:  

Year:  1982        PMID： 24186477     DOI： 10.1007/BF00435210

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  12 in total

1.  Presence of displacement loops in the covalently closed circular chloroplast deoxyribonucleic acid from higher plants.

Authors:  R Kolodner; K K Tewari
Journal:  J Biol Chem       Date:  1975-11-25       Impact factor: 5.157

2.  Synthesis and turnover of Euglena gracilis nuclear and chlorplast deoxyribonucleic acid.

Authors:  J E Manning; O C Richards
Journal:  Biochemistry       Date:  1972-05-23       Impact factor: 3.162

3.  Electron microscopic analysis of the extra 16 SrRNA gene and its neighbourhood in chloroplast DNA from Euglena gracilis strain Z.

Authors:  B Koller; H Delius
Journal:  FEBS Lett       Date:  1982-03-08       Impact factor: 4.124

4.  The chloroplast genome of bleached mutants of Euglena gracilis.

Authors:  P Heizmann; J Doly; Y Hussein; P Nicolas; V Nigon; G Bernardi
Journal:  Biochim Biophys Acta       Date:  1981-05-29

5.  The Euglena gracilis chloroplast genome: analysis by restriction enzymes.

Authors:  H Kopecka; E J Crouse; E Stutz
Journal:  Eur J Biochem       Date:  1977-02

6.  Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii.

Authors:  J D Rochaix; P Malnoe
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

7.  Are segments of chloroplast DNA differentially amplified?

Authors:  J R Mielenz; C L Hershberger
Journal:  Biochem Biophys Res Commun       Date:  1974-06-04       Impact factor: 3.575

8.  A novel closed circular duplex DNA in bleached mutant and green strains of Euglena gracilis.

Authors:  M M Nass; Y Ben-Shaul
Journal:  Biochim Biophys Acta       Date:  1972-06-22

9.  Influence of growth conditions upon the number of chloroplast DNA molecules in Euglena gracilis.

Authors:  J R Rawson; C Boerma
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

10.  Cellular content of chloroplast DNA and chloroplast ribosomal RNA genes in Euglena gracilis during chloroplast development.

Authors:  B K Chelm; P J Hoben; R B Hallick
Journal:  Biochemistry       Date:  1977-02-22       Impact factor: 3.162
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  3 in total

1.  Characterization of two circular plasmids from the marine diatom Cylindrotheca fusiformis: plasmids hybridize to chloroplast and nuclear DNA.

Authors:  J D Jacobs; J R Ludwig; M Hildebrand; A Kukel; T Y Feng; R W Ord; B E Volcani
Journal:  Mol Gen Genet       Date:  1992-05

2.  Red algal plasmids.

Authors:  L J Goff; A W Coleman
Journal:  Curr Genet       Date:  1990-12       Impact factor: 3.886

3.  A linear DNA molecule of 5.9 kilobase-pairs is highly homologous to the chloroplast DNA in the green alga Chlamydomonas moewusii.

Authors:  M Turmel; G Bellemare; R W Lee; C Lemieux
Journal:  Plant Mol Biol       Date:  1986-09       Impact factor: 4.076
  3 in total

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