Literature DB >> 24186232

Uptake of DNA by fragile mutants of Saccharomyces cerevisiae.

P V Venkov1, V P Ivanov.   

Abstract

When Saccharomyces cerevisiae SY15 rho° mutant cells grown in media stabilized with 10% sorbitol were suspended in 2% sorbitol solutions, 60-70% of the population did not lyse and became permeable to native high molecular weight DNA. Maximal incorporation of DNA to DNase resistant state was measured after 60 min of incubation in presence of 5 μg/ml DNA and 10 mM CaCl2. These results suggest that the fragile mutants might be tested as hosts for transformation of whole yeast cells.

Entities:  

Year:  1982        PMID： 24186232     DOI： 10.1007/BF00365707

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  9 in total

1.  Fusion of mitochondria with protoplasts in Saccharomyces cerevisiae.

Authors:  N Gunge; K Sakaguchi
Journal:  Mol Gen Genet       Date:  1979-03-05

2.  Saccharomyces cerevisiae: sorbitol-dependent fragile mutants.

Authors:  P V Venkov; A A Hadjiolov; E Battaner; D Schlessinger
Journal:  Biochem Biophys Res Commun       Date:  1974-02-04       Impact factor: 3.575

3.  The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.

Authors:  E S Goldring; L I Grossman; D Krupnick; D R Cryer; J Marmur
Journal:  J Mol Biol       Date:  1970-09-14       Impact factor: 5.469

4.  Rifampin susceptibility of ribonucleic acid synthesis in a fragile Saccharomyces cerevisiae mutant.

Authors:  P V Venkov; G I Milchev; A A Hadjiolov
Journal:  Antimicrob Agents Chemother       Date:  1975-12       Impact factor: 5.191

5.  Degradation of ribosomal precursor and polyadenylic acid-containing ribonucleic acids in Saccharomyces cerebisiae caused by actinomycin D.

Authors:  L W Waltschewa; P V Venkov; B B Stoyanova; A A Hadjiolov
Journal:  Arch Biochem Biophys       Date:  1976-10       Impact factor: 4.013

6.  Interactions of competent Streptococcus sanguis (Wicky) cells with native or denatured, homologous or heterologous deoxyribonucleic acids.

Authors:  J M Ranhand
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

7.  Toyocamycin inhibition of ribosomal ribonucleic acid processing in an osmotic-sensitive adenosine-utilizing Saccharomyces cerevisiae mutant.

Authors:  P V Venkov; L I Stateva; A A Hadjiolov
Journal:  Biochim Biophys Acta       Date:  1977-01-20

8.  Transformation of yeast.

Authors:  A Hinnen; J B Hicks; G R Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

9.  Macromolecule synthesis in temperature-sensitive mutants of yeast.

Authors:  L H Hartwell
Journal:  J Bacteriol       Date:  1967-05       Impact factor: 3.490
  9 in total

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