Production of heterokaryons of Candida albicans by protoplast fusions: Effects of differences in proportions and regenerative abilities of fusion partners.

Production of heterokaryons of Candida albicans by polyethylene glycol-induced fusion of complementing auxotrophic protoplasts was assayed in two series of fusion crosses designed to test the significances of the in-put ratios and regenerative abilities of parental protoplasts. The findings indicate that productive fusions require interaction of multiple protoplasts of each complementing type and can occur equally well with either regenerable or non-regenerable protoplasts.