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Literature DB >> 24185648

Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus.

Abstract

Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1-50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.

Entities: Chemical

Year: 1995 PMID： 24185648 DOI： 10.1007/BF01690247

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

12 in total

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Journal: Plant Physiol Date: 1986-06 Impact factor: 8.340

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Journal: Plant Cell Rep Date: 1987-07 Impact factor: 4.570

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Journal: Plant Cell Rep Date: 1988-06 Impact factor: 4.570

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Journal: Plant Cell Rep Date: 1989-10 Impact factor: 4.570

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Journal: Plant Cell Rep Date: 1991-04 Impact factor: 4.570

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Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

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Journal: Plant Physiol Date: 1983-11 Impact factor: 8.340

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Authors: S Ozcan; M Barghchi; S Firek; J Draper
Journal: Plant Cell Rep Date: 1992-02 Impact factor: 4.570

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Authors: K A Malik; P K Saxena
Journal: Planta Date: 1992-02 Impact factor: 4.116

3. Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Authors: Reza Azadi Gonbad; Uma Rani Sinniah; Maheran Abdul Aziz; Rosfarizan Mohamad
Journal: ScientificWorldJournal Date: 2014-01-28

3 in total