Sanaa Eissa1, Sherif M Shawky2, Marwa Matboli1, Shaymaa Mohamed1, Hassan M E Azzazy3. 1. Oncology Diagnostic Unit, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo 11381, Egypt. 2. Youssef Jameel Science & Technology Research Center, The American University in Cairo, P.O. Box 74, New Cairo 11835, Egypt. 3. Youssef Jameel Science & Technology Research Center, The American University in Cairo, P.O. Box 74, New Cairo 11835, Egypt; Department of Chemistry, The American University in Cairo, P.O. Box 74, New Cairo 11835, Egypt. Electronic address: hazzazy@aucegypt.edu.
Abstract
OBJECTIVE: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. DESIGN AND METHODS: HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. RESULTS: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. CONCLUSIONS: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.
OBJECTIVE: To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine. DESIGN AND METHODS: HURP RNA was extracted from urine samples (50 bladder carcinomapatients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles. RESULTS: The developed HURP RNA AuNP assay has a sensitivity and a specificity of 88.5% and 94%, respectively, and a detection limit of 2.4 nmol/L. The concordance between the HURP AuNP assay with RT-PCR after RNA purification using functionalized MNPs was 97%. CONCLUSIONS: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer.
Authors: Sherif M Shawky; Ahmed M Awad; Walaa Allam; Mohamed H Alkordi; Sherif F El-Khamisy Journal: Biosens Bioelectron Date: 2016-11-04 Impact factor: 10.618