| Literature DB >> 24182925 |
Abstract
Immunohistochemistry on free floating tissue sections is done for many reasons, all of which involve labeling tissue to visualize a certain cell type, protein, or structural component. Visualization is aided by mounting sections on microscope slides for stabilization, and is in most cases necessary for the appropriate use of objectives with a high numerical aperture and high degree of magnification.Keywords: Blocking nonspecific binding; Immunohistochemistry; Mount labeled tissue section; Pretreatment of tissue section; Primary antibody labeling; Secondary antibody labeling
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Year: 2013 PMID: 24182925 DOI: 10.1016/B978-0-12-420067-8.00013-1
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600