Literature DB >> 2417996

Propidium iodide staining of cytoautoradiographic preparations for the simultaneous determination of DNA content and grain count.

P A Giordano, G Mazzini, A Riccardi, C M Montecucco, G Ucci, M Danova.   

Abstract

A new method is described for staining cell nuclei with propidium iodide in preparations that have been processed for autoradiography. The permeability of the stripping film to the dye molecule has been studied, as have the staining conditions, in order to optimize the stoichiometry of the DNA-dye interaction. A procedure has also been set up to allow the simultaneous measurement of DNA content and grain count on the same cell.

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Year:  1985        PMID： 2417996     DOI： 10.1007/bf01002507

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  20 in total

1.  Methods of quantitative autoradiography using incident light microphotometry.

Authors:  P Dörmer; E Thiel
Journal:  J Histochem Cytochem       Date:  1976-01       Impact factor: 2.479

2.  An inexpensive microphotometer system for measuring silver grain densities in autoradiographs.

Authors:  H C Hughes; P M Meyer; J W Meyer; D R Meyer; J Bresnahan
Journal:  Stain Technol       Date:  1977-03

3.  Automated autoradiographic grain counting in human determined context.

Authors:  L E Lipkin; P Lemkin; G Carman
Journal:  J Histochem Cytochem       Date:  1974-07       Impact factor: 2.479

4.  Cytokinetics of subpopulations in mixed heteroploid tumors by television imaging. I. Deconvolution of the S-phase DNA ploidy composition. II. Analysis of the S-phase emptying profile of ploidy subpopulations.

Authors:  R J Sklarew
Journal:  J Histochem Cytochem       Date:  1984-04       Impact factor: 2.479

5.  Improvement in microscope photometry by voltage to frequency conversion: analogue measurement and digital processing.

Authors:  M I Freitas; P A Giordano; G Bottiroli
Journal:  J Microsc       Date:  1981-11       Impact factor: 1.758

6.  Computerized measurement of the DNA content, areas, and autoradiographic grains of the same nuclei: demonstration that lightly (3H)thymidine-labeled bone marrow cells are predominantly in G0/G1 and G2.

Authors:  D C Allison; J Meyne; P F Ridolpho; K Bose; M Chakerian; J Robertson
Journal:  J Histochem Cytochem       Date:  1984-11       Impact factor: 2.479

7.  Automated radioautographic grain counting. Correction for grain overlap.

Authors:  W H Schuette; S S Chen; S J Occhipinti; H S Mujagic; S E Shackney
Journal:  Cell Tissue Kinet       Date:  1983-05

8.  Simultaneous Feulgen densitometry and autoradiographic grain counting with the Quantimet 720D image-analysis system. I. Estimation of nuclear DNA content in 3H-thymidine-labeled cells.

Authors:  R J Sklarew
Journal:  J Histochem Cytochem       Date:  1982-01       Impact factor: 2.479

9.  Automated quantitative analysis of single and double label autoradiographs.

Authors:  A Rüter; H M Aus; H Harms; M Haucke; V ter Meulen; B Maurer-Schultze; H Korr; A Kellerer
Journal:  J Histochem Cytochem       Date:  1979-01       Impact factor: 2.479

Review 10.  Quantitative carbon-14 autoradiography at the cellular level: principles and application for cell kinetic studies.

Authors:  P Dörmer
Journal:  Histochem J       Date:  1981-03
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  2 in total

1.  Influence of cis-dichlorodiamineplatinum on glioma cell morphology and cell cycle kinetics in tissue culture.

Authors:  V Mares; P A Giordano; G Mazzini; V Lisá; C Pellicciari; E Scherini; G Bottiroli; J Drobnik
Journal:  Histochem J       Date:  1987-04

2.  Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes.

Authors:  Giuliano Mazzini; Marco Danova
Journal:  Methods Mol Biol       Date:  2023
  2 in total

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