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Literature DB >> 24178609

Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture.

R Sardana¹, S Dukiandjiev, M Giband, X Cheng, K Cowan, C Sauder, I Altosaar.

Abstract

The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.

Entities: Species

Year: 1996 PMID： 24178609 DOI： 10.1007/BF00231923

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

13 in total

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Journal: Theor Appl Genet Date: 1991-07 Impact factor: 5.699

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Journal: Plant Mol Biol Date: 1993-03 Impact factor: 4.076

6. Insect resistant rice generated by introduction of a modified delta-endotoxin gene of Bacillus thuringiensis.

Authors: H Fujimoto; K Itoh; M Yamamoto; J Kyozuka; K Shimamoto
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7. Synthetic cryIIIA gene from Bacillus thuringiensis improved for high expression in plants.

Authors: D W Sutton; P K Havstad; J D Kemp
Journal: Transgenic Res Date: 1992-09 Impact factor: 2.788

8. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation.

Authors: A H Christensen; R A Sharrock; P H Quail
Journal: Plant Mol Biol Date: 1992-02 Impact factor: 4.076

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Authors: F J Perlak; R W Deaton; T A Armstrong; R L Fuchs; S R Sims; J T Greenplate; D A Fischhoff
Journal: Biotechnology (N Y) Date: 1990-10

10. Characterization of a maize endosperm culture expressing zein genes and its use in transient transformation assays.

Authors: T J Quayle; W Hetz; G Feix
Journal: Plant Cell Rep Date: 1991-02 Impact factor: 4.570

14 in total

1. High-efficiency Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) and regeneration of insect-resistant transgenic plants.

Authors: Meenakshi Mehrotra; Indraneel Sanyal; D V Amla
Journal: Plant Cell Rep Date: 2011-04-23 Impact factor: 4.570

2. Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer.

Authors: X Cheng; R Sardana; H Kaplan; I Altosaar
Journal: Proc Natl Acad Sci U S A Date: 1998-03-17 Impact factor: 11.205

3. Molecular and functional characterization of cry1Ac transgenic pea lines.

Authors: Alemayehu Teressa Negawo; Linda Baranek; Hans-Jörg Jacobsen; Fathi Hassan
Journal: GM Crops Food Date: 2016-10 Impact factor: 3.074

4. Development of pod borer-resistant transgenic chickpea using a pod-specific and a constitutive promoter-driven fused cry1Ab/Ac gene.

Authors: Moumita Ganguly; Kutubuddin Ali Molla; Subhasis Karmakar; Karabi Datta; Swapan Kumar Datta
Journal: Theor Appl Genet Date: 2014-09-25 Impact factor: 5.699

5. Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus).

Authors: V Girijashankar; H C Sharma; Kiran K Sharma; V Swathisree; L Sivarama Prasad; B V Bhat; Monique Royer; Blanca San Secundo; M Lakshmi Narasu; I Altosaar; N Seetharama
Journal: Plant Cell Rep Date: 2005-09-20 Impact factor: 4.570

6. Biological activity of human granulocyte-macrophage colony stimulating factor is maintained in a fusion with seed glutelin peptide.

Authors: Ravinder K Sardana; Zaman Alli; Anil Dudani; Eilleen Tackaberry; Mitra Panahi; Muthukrishnan Narayanan; Peter Ganz; Illimar Altosaar
Journal: Transgenic Res Date: 2002-10 Impact factor: 2.788

7. Recombinant protein expression plasmids optimized for industrial E. coli fermentation and plant systems produce biologically active human insulin-like growth factor-1 in transgenic rice and tobacco plants.

Authors: Mitra Panahi; Zaman Alli; Xiongying Cheng; Loubaba Belbaraka; Jaafar Belgoudi; Ravinder Sardana; Jenny Phipps; Illimar Altosaar
Journal: Transgenic Res Date: 2004-06 Impact factor: 2.788

8. Transgenic rice plants expressing a modified cry1Ca1 gene are resistant to Spodoptera litura and Chilo suppressalis.

Authors: Mohsin Abbas Zaidi; Gongyin Ye; Hongwei Yao; Taek H You; Evelin Loit; Donald H Dean; Sheikh Riazuddin; Illimar Altosaar
Journal: Mol Biotechnol Date: 2009-11 Impact factor: 2.695

9. Expression of modified Cry1Ac gene of Bacillus thuringiensis in transgenic tobacco plants.

Authors: Lucyna Honorata Misztal; Adrianna Mostowska; Maria Skibinska; Joanna Bajsa; Wojciech Grzegorz Musial; Artur Jarmolowski
Journal: Mol Biotechnol Date: 2004-01 Impact factor: 2.695

10. Elite Indica transgenic rice plants expressing modified Cry1Ac endotoxin of Bacillus thuringiensis show enhanced resistance to yellow stem borer (Scirpophaga incertulas).

Authors: H K Khanna; S K Raina
Journal: Transgenic Res Date: 2002-08 Impact factor: 2.788