| Literature DB >> 24178562 |
Shozo Honda1, Yoriko Kirino, Yohei Kirino.
Abstract
Arginine methylation is an important posttranslational protein modification that modulates protein function for a wide range of biological processes. PIWI proteins, a subclade of the Argonaute family proteins, contain evolutionarily conserved symmetrical dimethylarginines (sDMAs). It has become increasingly apparent that the sDMAs of PIWI proteins serve as binding elements for TUDOR domain-containing proteins and that sDMA-dependent protein interactions play crucial roles in the biogenesis and function of PIWI-interacting RNAs (piRNAs). We describe a method for detecting PIWI sDMAs and purifying PIWI/piRNA complexes using anti-sDMA antibodies.Entities:
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Year: 2014 PMID： 24178562 PMCID： PMC4376280 DOI： 10.1007/978-1-62703-694-8_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745