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Literature DB >> 24178419

Two isoforms of acid phosphatase secreted by tobacco protoplasts: differential effect of brefeldin A on their secretion.

T S Kaneko¹, M Sato, M Osumi, M Muroi, A Takatsuki.

Abstract

The effects of brefeldin A (BFA) on the secretion of acid phosphatase (APase) by tobacco protoplasts were investigated. Secretion of APase was inhibited by BFA in a dose-dependent manner, with a concomitant intracellular accumulation of the enzyme. The secreted APase was composed of two isoforms. BFA (10/ μg/ml) inhibited the secretion of one of the isoforms without inhibiting that of the other, and this phenomenon explains the partial inhibition of APase secretion as a whole. The inhibition of APase secretion was accompanied by changes in the morphology of the Golgi apparatus and also by an increment in massdensity of cells.

Entities: Chemical Species

Year: 1996 PMID： 24178419 DOI： 10.1007/BF00232065

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

17 in total

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Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

Review 2. Intracellular trafficking of secretory proteins.

Authors: S Y Bednarek; N V Raikhel
Journal: Plant Mol Biol Date: 1992-10 Impact factor: 4.076

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Authors: B. Satiat-Jeunemaitre; C. Hawes
Journal: Plant Cell Date: 1994-04 Impact factor: 11.277

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Authors: J E Rothman; L Orci
Journal: Nature Date: 1992-01-30 Impact factor: 49.962

5. Plant protoplasts immobilized in calcium alginate: a simple method of preparing fragile cells for transmission electron microscopy.

Authors: K I Draget; S Myhre; K Evjen; K Ostgaard
Journal: Stain Technol Date: 1988-05

6. Breakdown of Ribulose Bisphosphate Carboxylase and Change in Proteolytic Activity during Dark-induced Senescence of Wheat Seedlings.

Authors: V A Wittenbach
Journal: Plant Physiol Date: 1978-10 Impact factor: 8.340

7. Selective inhibition of transcytosis by brefeldin A in MDCK cells.

Authors: W Hunziker; J A Whitney; I Mellman
Journal: Cell Date: 1991-11-01 Impact factor: 41.582

8. Secretion of the baby hamster kidney 30-kDa galactose-binding lectin from polarized and nonpolarized cells: a pathway independent of the endoplasmic reticulum-Golgi complex.

Authors: S Sato; I Burdett; R C Hughes
Journal: Exp Cell Res Date: 1993-07 Impact factor: 3.905