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Literature DB >> 24177917

Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae.

P Breining¹, A P Surguchov, W Piepersberg.

Abstract

A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).

Entities: Gene Species

Year: 1984 PMID： 24177917 DOI： 10.1007/BF00433913

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

17 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

Review 2. Suppressors in yeast.

Authors: D C Hawthorne; U Leupold
Journal: Curr Top Microbiol Immunol Date: 1974 Impact factor: 4.291

10. Further characterization of recessive suppression in yeast. Isolation of the low-temperature sensitive mutant of Saccharomyces cerevisiae defective in the assembly of 60 S ribosomal subunit.

Authors: A P Surguchov; E S Fominykch; V N Smirnov; M D Ter-Avanesyan; L N Mironova; S G Inge-Vechtomov
Journal: Biochim Biophys Acta Date: 1981-06-26

2 in total

1. Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae.

Authors: A B Sudarickov; A P Surguchov
Journal: Mol Gen Genet Date: 1986-05

Review 2. Polypeptide chain termination in Saccharomyces cerevisiae.

Authors: I Stansfield; M F Tuite
Journal: Curr Genet Date: 1994-05 Impact factor: 3.886

2 in total

Cloning and identification of a DNA fragment coding for the sup1 gene of Saccharomyces cerevisiae.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Review 2. Suppressors in yeast.

3. A small cosmid for efficient cloning of large DNA fragments.

4. DNA rearrangements associated with a transposable element in yeast.

5. Rapid procedure for detection and isolation of large and small plasmids.

6. Genetic map of Saccharomyces cerevisiae.

7. Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation.

8. The structure of transposable yeast mating type loci.

9. Synergistic action of genetic and phenotypic suppression of nonsense mutations in yeast Saccharomyces cerevisiae.

10. Further characterization of recessive suppression in yeast. Isolation of the low-temperature sensitive mutant of Saccharomyces cerevisiae defective in the assembly of 60 S ribosomal subunit.

1. Immunologically related proteins in cytoplasmic and mitochondrial ribosomes of yeast Saccharomyces cerevisiae.

Review 2. Polypeptide chain termination in Saccharomyces cerevisiae.