Literature DB >> 24173569

Multicolour whole-mount in situ hybridization to Drosophila embryos.

G Hauptmann1, T Gerster.   

Abstract

We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.

Entities:  

Year:  1996        PMID： 24173569     DOI： 10.1007/s004270050055

Source DB:  PubMed          Journal:  Dev Genes Evol        ISSN： 0949-944X            Impact factor:   0.900


  7 in total

1.  The Drosophila homolog of the human AF10 is an HP1-interacting suppressor of position effect variegation.

Authors:  B Linder; N Gerlach; H Jäckle
Journal:  EMBO Rep       Date:  2001-03       Impact factor: 8.807

2.  Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos.

Authors:  Giselbert Hauptmann; Iris Söll; Robert Krautz; Ulrich Theopold
Journal:  J Vis Exp       Date:  2016-01-31       Impact factor: 1.355

3.  Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions.

Authors:  Pilar Carrera; Yuri M Moshkin; Sebastian Gronke; Herman H W Sillje; Erich A Nigg; Herbert Jackle; Francois Karch
Journal:  Genes Dev       Date:  2003-10-15       Impact factor: 11.361

4.  Formaldehyde-based whole-mount in situ hybridization method for planarians.

Authors:  Bret J Pearson; George T Eisenhoffer; Kyle A Gurley; Jochen C Rink; Diane E Miller; Alejandro Sánchez Alvarado
Journal:  Dev Dyn       Date:  2009-02       Impact factor: 3.780

5.  Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain.

Authors:  Gilbert Lauter; Iris Söll; Giselbert Hauptmann
Journal:  Neural Dev       Date:  2011-04-05       Impact factor: 3.842

6.  Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems.

Authors:  Gilbert Lauter; Iris Söll; Giselbert Hauptmann
Journal:  BMC Dev Biol       Date:  2011-07-04       Impact factor: 1.978

7.  Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos.

Authors:  Theresa Gross-Thebing; Azadeh Paksa; Erez Raz
Journal:  BMC Biol       Date:  2014-08-15       Impact factor: 7.431
  7 in total

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