Literature DB >> 24173381

The need for transparency and good practices in the qPCR literature.

Stephen A Bustin1, Vladimir Benes, Jeremy Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory Shipley, Carl T Wittwer, Peter Schjerling, Philip J Day, Mónica Abreu, Begoña Aguado, Jean-François Beaulieu, Anneleen Beckers, Sara Bogaert, John A Browne, Fernando Carrasco-Ramiro, Liesbeth Ceelen, Kate Ciborowski, Pieter Cornillie, Stephanie Coulon, Ann Cuypers, Sara De Brouwer, Leentje De Ceuninck, Jurgen De Craene, Hélène De Naeyer, Ward De Spiegelaere, Kato Deckers, Annelies Dheedene, Kaat Durinck, Margarida Ferreira-Teixeira, Annelies Fieuw, Jack M Gallup, Sandra Gonzalo-Flores, Karen Goossens, Femke Heindryckx, Elizabeth Herring, Hans Hoenicka, Laura Icardi, Rolf Jaggi, Farzad Javad, Michael Karampelias, Frederick Kibenge, Molly Kibenge, Candy Kumps, Irina Lambertz, Tim Lammens, Amelia Markey, Peter Messiaen, Evelien Mets, Sofia Morais, Alberto Mudarra-Rubio, Justine Nakiwala, Hilde Nelis, Pal A Olsvik, Claudina Pérez-Novo, Michelle Plusquin, Tony Remans, Ali Rihani, Paulo Rodrigues-Santos, Pieter Rondou, Rebecca Sanders, Katharina Schmidt-Bleek, Kerstin Skovgaard, Karen Smeets, Laura Tabera, Stefan Toegel, Tim Van Acker, Wim Van den Broeck, Joni Van der Meulen, Mireille Van Gele, Gert Van Peer, Mario Van Poucke, Nadine Van Roy, Sarah Vergult, Joris Wauman, Marina Tshuikina-Wiklander, Erik Willems, Sara Zaccara, Fjoralba Zeka, Jo Vandesompele.   

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Mesh:

Year:  2013        PMID: 24173381     DOI: 10.1038/nmeth.2697

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


  22 in total

1.  Improving validation practices in "omics" research.

Authors:  John P A Ioannidis; Muin J Khoury
Journal:  Science       Date:  2011-12-02       Impact factor: 47.728

2.  Improving the analysis of quantitative PCR data in veterinary research.

Authors:  Stephen Bustin; Louis C Penning
Journal:  Vet J       Date:  2011-10-17       Impact factor: 2.688

Review 3.  RNA integrity and the effect on the real-time qRT-PCR performance.

Authors:  Simone Fleige; Michael W Pfaffl
Journal:  Mol Aspects Med       Date:  2006-02-15

Review 4.  The real-time polymerase chain reaction.

Authors:  Mikael Kubista; José Manuel Andrade; Martin Bengtsson; Amin Forootan; Jiri Jonák; Kristina Lind; Radek Sindelka; Robert Sjöback; Björn Sjögreen; Linda Strömbom; Anders Ståhlberg; Neven Zoric
Journal:  Mol Aspects Med       Date:  2006-02-03

5.  How to do successful gene expression analysis using real-time PCR.

Authors:  Stefaan Derveaux; Jo Vandesompele; Jan Hellemans
Journal:  Methods       Date:  2009-12-05       Impact factor: 3.608

6.  Primer sequence disclosure: a clarification of the MIQE guidelines.

Authors:  Stephen A Bustin; Vladimir Benes; Jeremy A Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory L Shipley; Jo Vandesompele; Carl T Wittwer
Journal:  Clin Chem       Date:  2011-03-18       Impact factor: 8.327

7.  Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

Authors:  Jeremy A Garson; Jim F Huggett; Stephen A Bustin; Michael W Pfaffl; Vladimir Benes; Jo Vandesompele; Gregory L Shipley
Journal:  AIDS Res Hum Retroviruses       Date:  2009-03       Impact factor: 2.205

8.  MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments.

Authors:  Stephen A Bustin; Jean-François Beaulieu; Jim Huggett; Rolf Jaggi; Frederick S B Kibenge; Pål A Olsvik; Louis C Penning; Stefan Toegel
Journal:  BMC Mol Biol       Date:  2010-09-21       Impact factor: 2.946

9.  Transparency of reporting in molecular diagnostics.

Authors:  Stephen Bustin
Journal:  Int J Mol Sci       Date:  2013-07-30       Impact factor: 5.923

10.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583

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Journal:  Nat Genet       Date:  2014-05-25       Impact factor: 38.330

2.  Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy.

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Journal:  Plant Cell       Date:  2014-10-31       Impact factor: 11.277

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Authors:  G Bruckert; D Vivien; F Docagne; B D Roussel
Journal:  Mol Neurobiol       Date:  2015-02-07       Impact factor: 5.590

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Journal:  Mol Neurobiol       Date:  2015-10-17       Impact factor: 5.590

Review 5.  Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?

Authors:  J R Dijkstra; L C van Kempen; I D Nagtegaal; S A Bustin
Journal:  Mol Oncol       Date:  2014-01-02       Impact factor: 6.603

Review 6.  Diagnostic molecular techniques in haematology: recent advances.

Authors:  Aikaterini Koutsi; Elisavet-Christina Vervesou
Journal:  Ann Transl Med       Date:  2018-06

7.  Prevention of unpredictable chronic stress-related phenomena in zebrafish exposed to bromazepam, fluoxetine and nortriptyline.

Authors:  Matheus Marcon; Ana P Herrmann; Ricieri Mocelin; Cassiano L Rambo; Gessi Koakoski; Murilo S Abreu; Greicy M M Conterato; Luiza W Kist; Maurício R Bogo; Leila Zanatta; Leonardo J G Barcellos; Angelo L Piato
Journal:  Psychopharmacology (Berl)       Date:  2016-08-25       Impact factor: 4.530

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9.  Appropriate real-time PCR reference genes for fluoride treatment studies performed in vitro or in vivo.

Authors:  D Faibish; M Suzuki; J D Bartlett
Journal:  Arch Oral Biol       Date:  2015-11-10       Impact factor: 2.633

10.  MicroRNA-196a2 Biomarker and Targetome Network Analysis in Solid Tumors.

Authors:  Eman A Toraih; Manal S Fawzy; Eman A Mohammed; Mohammad H Hussein; Mohamad M El-Labban
Journal:  Mol Diagn Ther       Date:  2016-12       Impact factor: 4.074

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