Effectiveness of denture cleanser associated with microwave disinfection and brushing of complete dentures: in vivo study.

Complete dentures acts as a reservoir for microbial colonization, which may lead to systemic infections. Microwave irradiation has been used as an efficient method of denture disinfection. Even though current methods eliminate denture-base microorganisms, a recurrence rate of denture stomatitis (DS) is still observed among denture-wearing patients. It was hypothesized that microwave disinfection kills microorganisms but do not remove dead bacteria from the denture surface. To test this hypothesis, the biofilm found in the dentures of 10 patients with DS was evaluated. In addition, the effects of microwave irradiation plus brushing (MW+B) on the denture biofilm and the combination of denture cleanser with microwave irradiation and brushing (MW+DC+B) for the removal of denture-accumulating microorganisms were investigated. Microbiological data were analyzed statistically by nonparametric analysis (Friedman/Wilcoxon, α=0.05). MW+B and MW+DC+B were effective in reducing the rate of microorganisms (99.2% and 99.5% respectively), but without significant difference between them (p=0.553). However, it was observed that the complete removal of microorganisms from denture surface was only possible when all regimens were combined (MW+DC+B). Microwave irradiation in combination with soaking in denture cleanser and brushing effectively disinfected the dentures and removed denture biofilm.