| Literature DB >> 2416597 |
Abstract
The affinity of epidermolytic toxin from Staphylococcus aureus for proteins from the target tissue has been tested by a Western blotting procedure. Particular proteins in a 1 M phosphate extract of epidermis reacted on nitrocellulose blots with a probe prepared by the conjugation of toxin with peroxidase. Protein extracted into 50 mM Tris-HCl did not react. The probe detected profilaggrin, filaggrin and a smaller unidentified polypeptide. It is suggested that the interaction is relevant to the mode of action of the toxin.Entities:
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Year: 1986 PMID： 2416597 DOI： 10.1016/0014-5793(86)80107-7
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124