Linkage mapping of prolamin and isozyme genes on the 1S(l) chromosome of Aegilops longissima.

The storage proteins and isozymes of two accessions of Aegilops longissima, and the F2 progeny from the cross between them, were analyzed. Six loci were identified on the 1S(l) chromosome: Glu-S (l) 1 (coding for HMW subunits of glutenin), Gpi-S (l) 1 (coding for a Gpi isozyme), Glu-S (l) 3 (coding for LMW subunits of glutenin), Gli-S (l) 1 (coding for gliadins) and two, so far, not described new loci Gli-S (l) 4 and Gli-S (l) 5. The Gli-S (l) 4 locus codes for a ω-gliadin and the Gli-S (l) 5 codes for a gliadin with mobility in the β-region. The genetical distances found between the six loci allowed the establishment of the following gene order on the 1S(l) chromosome: Glu-S (l) 1 -centromere -Gpi-S (l) 1 - Gli-S (l) 4 - Gli-S (l) 3 - Gli-S (l) 1 -Gli-S (l) 5.