Literature DB >> 24161897

Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant.

Nest McKain1, Buğra Genc, Timothy J Snelling, R John Wallace.   

Abstract

Bacteria and archaea in frozen (-20°C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected.
© 2013.

Entities:  

Keywords:  Cryoprotection; Glycerol; Rumen; Storage

Mesh：

Substances：

Year:  2013        PMID： 24161897     DOI： 10.1016/j.mimet.2013.10.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


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