Literature DB >> 2416116

Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions.

A D Hoffman, B Banapour, J A Levy.   

Abstract

The RNA-dependent DNA polymerase of the AIDS-associated retrovirus (ARV) gives highest activity with the synthetic template, poly(rA)oligo(dT) and prefers Mg2+ over Mn2+ as a divalent cation. It can use other template-primer combinations but with poly(rCm)oligo(dG), it prefers Mn2+ over Mg2+. Detection of ARV reverse transcriptase in culture fluids is substantially increased with an optimal KCl concentration and a special combination of EGTA and reducing agents. Our results indicate some distinguishing characteristics of the ARV reverse transcriptase and optimal conditions for its detection.

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Year:  1985        PMID： 2416116     DOI： 10.1016/0042-6822(85)90135-7

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  123 in total

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9.  Mechanism of resistance of human immunodeficiency virus type 1 to 2',3'-dideoxyinosine.

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