Literature DB >> 2415643

A new common polysaccharide antigen of strains of Pseudomonas aeruginosa detected with a monoclonal antibody.

S Sawada, T Kawamura, Y Masuho, K Tomibe.   

Abstract

A monoclonal antibody, E87, that binds to various serotype strains of Pseudomonas aeruginosa was produced by a hybridoma cell line prepared by fusion of mouse plasmacytoma P3-X63-Ag8-U1 with spleen cells of mice immunized with P. aeruginosa strain IFO3080 (serotype M). E87 bound to approximately 80% of the tested strains of various serotypes of P. aeruginosa. The antigen recognized with E87 was found in the lipopolysaccharide and was eluted in void volume fractions of Sephadex G50 column chromatography after acetic acid treatment. This antigen was eluted in the fraction containing substances of lower molecular weight than the O side chain by Sephacryl S-300 column chromatography, and this fraction was found to be the peak fraction of hexose. This antigen was not separated from the O side chain by Sephacryl S-300 column chromatography of untreated lipopolysaccharide. Colorimetric analyses and thin-layer chromatography showed that this antigen consisted mainly of rhamnose and ribose (molar ratio, 10:1).

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Year:  1985        PMID： 2415643     DOI： 10.1093/infdis/152.6.1290

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  12 in total

1.  Common antigen lipopolysaccharide from Pseudomonas aeruginosa AK1401 as a receptor for bacteriophage A7.

Authors:  M Rivera; T R Chivers; J S Lam; E J McGroarty
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

2.  Growth-dependent alterations in production of serotype-specific and common antigen lipopolysaccharides in Pseudomonas aeruginosa PAO1.

Authors:  E J McGroarty; M Rivera
Journal:  Infect Immun       Date:  1990-04       Impact factor: 3.441

3.  Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa.

Authors:  M Y Lam; E J McGroarty; A M Kropinski; L A MacDonald; S S Pedersen; N Høiby; J S Lam
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

4.  Occurrence of D-rhamnan as the common antigen reactive against monoclonal antibody E87 in Pseudomonas aeruginosa IFO 3080 and other strains.

Authors:  S Yokota; S Kaya; Y Araki; E Ito; T Kawamura; S Sawada
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

Review 5.  Genetics of O-antigen biosynthesis in Pseudomonas aeruginosa.

Authors:  H L Rocchetta; L L Burrows; J S Lam
Journal:  Microbiol Mol Biol Rev       Date:  1999-09       Impact factor: 11.056

6.  Pseudomonas aeruginosa lipopolysaccharide: evidence that the O side chains and common antigens are on the same molecule.

Authors:  K Hatano; J B Goldberg; G B Pier
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

7.  Synthesis of lipopolysaccharide O side chains by Pseudomonas aeruginosa PAO1 requires the enzyme phosphomannomutase.

Authors:  J B Goldberg; K Hatano; G B Pier
Journal:  J Bacteriol       Date:  1993-03       Impact factor: 3.490

8.  Analysis of a common-antigen lipopolysaccharide from Pseudomonas aeruginosa.

Authors:  M Rivera; E J McGroarty
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

9.  Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, phi CTX, of Pseudomonas aeruginosa.

Authors:  S Yokota; T Hayashi; H Matsumoto
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

10.  Production and characterization of monoclonal antibodies against serotype strains of Pseudomonas aeruginosa.

Authors:  J S Lam; L A MacDonald; M Y Lam; L G Duchesne; G G Southam
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441
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