| Literature DB >> 24155234 |
Abstract
HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.Entities:
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Year: 2014 PMID: 24155234 DOI: 10.1007/7651_2013_42
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745