Evgeny A Reshetnikov1, Ludmila Y Akulova2, Irina S Dobrodomova2, Volodymyr Y Dvornyk3, Alexey V Polonikov4, Mikhail I Churnosov2. 1. Department of Medical Biological Disciplines, Belgorod National Research University, Russia reshetnikov@bsu.edu.ru. 2. Department of Medical Biological Disciplines, Belgorod National Research University, Russia. 3. School of Biological Sciences, University of Hong Kong, China. 4. Department of Biology, Medical Genetics and Ecology, Kursk State Medical University, Russia.
Abstract
INTRODUCTION: Malfunctioning of the cardiovascular system during pregnancy may be responsible for adverse effects on the 'mother-fetus' system. The cardiovascular system of a pregnant woman develops adaptation to the increased load. Angiotensin-converting enzyme (ACE) is known to play an important role in the adaptation. The present study was designed to investigate whether the insertion-deletion (I/D) polymorphism of the ACE gene is associated with the level of arterial blood pressure in women before and during pregnancy. MATERIALS AND METHODS: The level of blood pressure was measured in 591 Russian women (Central Russia) before and during (37-40 weeks term) pregnancy. The women were divided into three groups which were hypertensive, hypotensive, and normotensive according to blood pressure level. Genotyping of the ACE I/D polymorphism was performed using polymerase chain reaction (PCR) and amplified fragment length polymorphism assay. RESULTS: Women with genotype DD showed the highest blood pressure level both during and at the end of pregnancy (p<0.05). The highest frequencies of allele D and genotype DD were found in pregnant women in the hypertensive group. CONCLUSIONS: The deletion variant of the ACE gene is associated with high blood pressure level at the end of pregnancy.
INTRODUCTION: Malfunctioning of the cardiovascular system during pregnancy may be responsible for adverse effects on the 'mother-fetus' system. The cardiovascular system of a pregnant woman develops adaptation to the increased load. Angiotensin-converting enzyme (ACE) is known to play an important role in the adaptation. The present study was designed to investigate whether the insertion-deletion (I/D) polymorphism of the ACE gene is associated with the level of arterial blood pressure in women before and during pregnancy. MATERIALS AND METHODS: The level of blood pressure was measured in 591 Russian women (Central Russia) before and during (37-40 weeks term) pregnancy. The women were divided into three groups which were hypertensive, hypotensive, and normotensive according to blood pressure level. Genotyping of the ACE I/D polymorphism was performed using polymerase chain reaction (PCR) and amplified fragment length polymorphism assay. RESULTS:Women with genotype DD showed the highest blood pressure level both during and at the end of pregnancy (p<0.05). The highest frequencies of allele D and genotype DD were found in pregnant women in the hypertensive group. CONCLUSIONS: The deletion variant of the ACE gene is associated with high blood pressure level at the end of pregnancy.
Authors: Luciana Neves Cosenso-Martin; Renan Oliveira Vaz-de-Melo; Luana Rocco Pereira; Cláudia Bernardi Cesarino; Juan Carlos Yugar-Toledo; José Paulo Cipullo; Marcela Augusta de Souza Pinhel; Dorotéia Rossi Silva Souza; José Fernando Vilela-Martin Journal: Eur J Med Res Date: 2015-09-04 Impact factor: 2.175