Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A specific iron stain for iron-binding proteins in polyacrylamide gels: application to transferrin and lactoferrin.

Literature DB >> 2415022

A specific iron stain for iron-binding proteins in polyacrylamide gels: application to transferrin and lactoferrin.

Abstract

A new method for specifically staining the iron atoms present in transferrin and lactoferrin after polyacrylamide gel electrophoresis and isoelectric focusing is described. The stain, 3-(2-pyridyl)-5,6-bis(2-(5-furylsulfonic acid))-1,2,4-triazine, disodium salt, or Ferene S, will detect transferrin in 5 microliters of human serum, lactoferrin in 10 microliters of human whey, and 10 micrograms of purified primate (Macaca fascicularis) transferrin. This method of staining is very rapid as the serum transferrin bands can be seen within 5 to 10 min of staining.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1985 PMID： 2415022 DOI： 10.1016/0003-2697(85)90258-1

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

Keyword Cloud
Cited

18 in total

1. An immunoreactive 38-kilodalton protein of Ehrlichia canis shares structural homology and iron-binding capacity with the ferric ion-binding protein family.

Authors: C Kuyler Doyle; Xiaofeng Zhang; Vsevolod L Popov; Jere W McBride
Journal: Infect Immun Date: 2005-01 Impact factor: 3.441

2. The Brachyspira hyodysenteriae ftnA gene: DNA vaccination and real-time PCR quantification of bacteria in a mouse model of disease.

Authors: Antony J Davis; Stuart C Smith; Robert J Moore
Journal: Curr Microbiol Date: 2005-06-13 Impact factor: 2.188

3. An iron-binding protein, Dpr, decreases hydrogen peroxide stress and protects Streptococcus pyogenes against multiple stresses.

Authors: Chih-Cheng Tsou; Chuan Chiang-Ni; Yee-Shin Lin; Woei-Jer Chuang; Ming-T Lin; Ching-Chuan Liu; Jiunn-Jong Wu
Journal: Infect Immun Date: 2008-06-09 Impact factor: 3.441

4. Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

Authors: Victoria J Gauci; Elise P Wright; Jens R Coorssen
Journal: J Chem Biol Date: 2010-06-19

5. Inhibiting the BfrB:Bfd interaction in Pseudomonas aeruginosa causes irreversible iron accumulation in bacterioferritin and iron deficiency in the bacterial cytosol.

Authors: Kate Eshelman; Huili Yao; Achala N D Punchi Hewage; Jacqueline J Deay; Josephine R Chandler; Mario Rivera
Journal: Metallomics Date: 2017-06-21 Impact factor: 4.526

10. Isolation, purification and characterization of an iron-binding protein from the horseshoe crab (Limulus polyphemus).

Authors: R Topham; B Cooper; S Tesh; G Godette; C Bonaventura; J Bonaventura
Journal: Biochem J Date: 1988-05-15 Impact factor: 3.857

A specific iron stain for iron-binding proteins in polyacrylamide gels: application to transferrin and lactoferrin.

1. An immunoreactive 38-kilodalton protein of Ehrlichia canis shares structural homology and iron-binding capacity with the ferric ion-binding protein family.

2. The Brachyspira hyodysenteriae ftnA gene: DNA vaccination and real-time PCR quantification of bacteria in a mouse model of disease.

3. An iron-binding protein, Dpr, decreases hydrogen peroxide stress and protects Streptococcus pyogenes against multiple stresses.

4. Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

5. Inhibiting the BfrB:Bfd interaction in Pseudomonas aeruginosa causes irreversible iron accumulation in bacterioferritin and iron deficiency in the bacterial cytosol.

6. Effect of nonheme iron-containing ferritin Dpr in the stress response and virulence of pneumococci.

7. Ferritin from the haemolymph of adult ants: an extraction method for characterization and a ferromagnetic study.

8. An iron-binding protein, Dpr, from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro.

9. The iron-binding protein Dps confers hydrogen peroxide stress resistance to Campylobacter jejuni.

10. Isolation, purification and characterization of an iron-binding protein from the horseshoe crab (Limulus polyphemus).