Literature DB >> 2415020

A comparison of silver staining methods for detecting proteins in ultrathin polyacrylamide gels on support film after isoelectric focusing.

L A Butcher, J K Tomkins.   

Abstract

The application of silver staining methods to the detection of proteins on ultrathin isoelectric focusing gel systems requires the optimization of many steps in the procedure in order to obtain reproducible staining of proteins with acceptable levels of background. Three different methods which have been reported for detecting proteins by silver staining in sodium dodecyl sulfate-polyacrylamide gel systems were investigated. A major problem with staining ultrathin isoelectric focusing gels was found to be surface staining that was associated with gels cast on support films. A modification of the method of Poehling and Neuhoff (H.-M. Poehling and V. Neuhoff, 1981, Electrophoresis 2, 141-147) was found to give the best results.

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Year:  1985        PMID： 2415020     DOI： 10.1016/0003-2697(85)90243-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Distribution of glucose/mannose-specific isolectins in pea (Pisum sativum L.) seedlings.

Authors:  C L Díaz; M Hosselet; G J Logman; E van Driessche; B J Lugtenberg; J W Kijne
Journal:  Planta       Date:  1990-07       Impact factor: 4.116

2.  Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of beta-D-glucose.

Authors:  S Witt; G Wohlfahrt; D Schomburg; H J Hecht; H M Kalisz
Journal:  Biochem J       Date:  2000-04-15       Impact factor: 3.857

3.  Purification and biochemical characterization of a novel thermostable lipase from Aspergillus niger.

Authors:  V M Namboodiri; R Chattopadhyaya
Journal:  Lipids       Date:  2000-05       Impact factor: 1.880

4.  Purification and partial characterization of the Rhizobium leguminosarum biovar viciae Ca2+-dependent adhesin, which mediates the first step in attachment of cells of the family Rhizobiaceae to plant root hair tips.

Authors:  G Smit; T J Logman; M E Boerrigter; J W Kijne; B J Lugtenberg
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

5.  Structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase expressed in Escherichia coli.

Authors:  S Witt; M Singh; H M Kalisz
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

6.  Modification in Silver Staining Procedure for Enhanced Protein Staining.

Authors:  Yibo Jiang; LinLin Zheng; Lulin Lin; Shan Lin; Kui Xu; SiJie Deng; QiQing Zhang
Journal:  Biomed Res Int       Date:  2022-05-13       Impact factor: 3.246

7.  A major binding protein for leukemia inhibitory factor in normal mouse serum: identification as a soluble form of the cellular receptor.

Authors:  M J Layton; B A Cross; D Metcalf; L D Ward; R J Simpson; N A Nicola
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

8.  Inter-species chimeras of leukaemia inhibitory factor define a major human receptor-binding determinant.

Authors:  C M Owczarek; M J Layton; D Metcalf; P Lock; T A Willson; N M Gough; N A Nicola
Journal:  EMBO J       Date:  1993-09       Impact factor: 11.598
  8 in total

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