| Literature DB >> 24148243 |
Jingting Li1, Kirk C Hansen, Ying Zhang, Chenbo Dong, Cerasela Z Dinu, Monika Dzieciatkowska, Ming Pei.
Abstract
Autologous cells suffer from limited cell number and senescence during ex vivo expansion for cartilage repair. Here we found that expansion on extracellular matrix (ECM) deposited by fetal synovium-derived stem cells (SDSCs) (FE) was superior to ECM deposited by adult SDSCs (AE) in promoting cell proliferation and chondrogenic potential. Unique proteins in FE might be responsible for the rejuvenation effect of FE while advantageous proteins in AE might contribute to differentiation more than to proliferation. Compared to AE, the lower elasticity of FE yielded expanded adult SDSCs with lower elasticity which could be responsible for the enhancement of chondrogenic and adipogenic differentiation. MAPK and noncanonical Wnt signals were actively involved in ECM-mediated adult SDSC rejuvenation.Entities:
Keywords: Cell senescence; Chondrogenesis; Decellularized stem cell matrix; Fetal stem cell; Monolayer expansion; Synovium-derived stem cell
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Year: 2013 PMID： 24148243 DOI： 10.1016/j.biomaterials.2013.09.099
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479