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Literature DB >> 2414719

A microscope stage temperature controller for the study of whole-cell or single-channel currents.

L D Chabala, R E Sheridan, D C Hodge, J N Power, M P Walsh.

Abstract

The construction of a microscope stage temperature controller is described that works equally well with upright or inverted microscopes. The control circuit directly regulates the bath temperature near the physiological preparation from 0.0 degree C to 40.0 degrees C with a stability of +/- 0.1 degrees C; it allows biophysical studies of whole-cell or single channel currents to be carried out at different temperatures without introducing additional electrical noise into the measurements. The device may also be of interest to neurobiologists who work with tissue-culture preparations.

Mesh：

Substances：
Ion Channels

Year: 1985 PMID： 2414719 DOI： 10.1007/bf00585351

Source DB: PubMed Journal: Pflugers Arch ISSN： 0031-6768 Impact factor: 3.657

4 in total

1. A transition state theory approach to the kinetics of conductance changes in excitable membranes.

Authors: R W Tsien; D Noble
Journal: J Membr Biol Date: 1969-12 Impact factor: 1.843

2. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

Authors: O P Hamill; A Marty; E Neher; B Sakmann; F J Sigworth
Journal: Pflugers Arch Date: 1981-08 Impact factor: 3.657

3. Photoactivation and dissociation of agonist molecules at the nicotinic acetylcholine receptor in voltage-clamped rat myoballs.

Authors: L D Chabala; A M Gurney; H A Lester
Journal: Biophys J Date: 1985-08 Impact factor: 4.033

4. Photoinduced removal of nifedipine reveals mechanisms of calcium antagonist action on single heart cells.

Authors: A M Gurney; J M Nerbonne; H A Lester
Journal: J Gen Physiol Date: 1985-09 Impact factor: 4.086

4 in total

7 in total

1. Precise and millidegree stable temperature control for fluorescence imaging: application to phase transitions in lipid membranes.

Authors: Elaine R Farkas; Watt W Webb
Journal: Rev Sci Instrum Date: 2010-09 Impact factor: 1.523

6. Single channel properties of the N-methyl-D-aspartate receptor channel using NMDA and NMDA agonists: on-cell recordings.

Authors: J G McLarnon; K Curry
Journal: Exp Brain Res Date: 1990 Impact factor: 1.972

7. An optofluidic temperature probe.

Authors: Ilona Węgrzyn; Alar Ainla; Gavin David Michael Jeffries; Aldo Jesorka
Journal: Sensors (Basel) Date: 2013-03-28 Impact factor: 3.576

7 in total

A microscope stage temperature controller for the study of whole-cell or single-channel currents.

1. A transition state theory approach to the kinetics of conductance changes in excitable membranes.

2. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.

3. Photoactivation and dissociation of agonist molecules at the nicotinic acetylcholine receptor in voltage-clamped rat myoballs.

4. Photoinduced removal of nifedipine reveals mechanisms of calcium antagonist action on single heart cells.

1. Precise and millidegree stable temperature control for fluorescence imaging: application to phase transitions in lipid membranes.

2. Temperature dependence of drug blockade of a calcium-dependent potassium channel in cultured hippocampal neurons.

3. Dose-response of acetylcholine receptor channels opened by a flash-activated agonist in voltage-clamped rat myoballs.

4. Analysis of electrophysiological properties and responses of neutrophils.

5. Temperature dependence of unitary properties of an ATP-dependent potassium channel in cardiac myocytes.

6. Single channel properties of the N-methyl-D-aspartate receptor channel using NMDA and NMDA agonists: on-cell recordings.

7. An optofluidic temperature probe.