| Literature DB >> 24145153 |
Liming Zhu1, Xiaojiao Cheng2, Yanfei Ding2, Jindong Shi3, Huanyu Jin3, Hong Wang3, Yunlin Wu2, Jing Ye4, Yiming Lu4, Timothy C Wang5, Chung S Yang3, Shui Ping Tu6.
Abstract
Bone marrow-derived myofibroblasts (BMFs) have been shown to promote tumor growth. Here, we found that BMFs or BMF conditioned medium (BMF-CM) induced cancer stem cell-like sphere formation of colon cancer cells. The co-cultured BMFs, but not co-cultured cancer cells, expressed higher levels of IL-6 than BMFs or cancer cells cultured alone. Anti-mouse IL-6 neutralizing antibody, JAK2 inhibitors and STAT3 knockdown in mouse cancer cells reduced BMF- and BMF-CM-induced sphere formation of colon cancer cells. When co-injected, BMFs significantly enhanced tumorigenesis of colon cancer cells in mice. Our results demonstrate that BMFs promote tumorigenesis via the activation of the IL-6/JAK2/STAT3 pathway.Entities:
Keywords: BMF conditioned medium; BMF-CM; BMFs; Bone marrow-derived myofibroblasts; CAFs; Cancer stem cell; Co-culture-CM; Colon cancer; EGF; FL; IL-6; JAK2; MSCs; STAT3; TGF-β; bFGF; basic fibroblast growth factor; bone marrow-derived myofibroblasts; cancer-associated fibroblasts; co-culture medium of BMFs and cancer cells; epithelial growth factor; firefly luciferase; interleukin-6; janus kinase 2; mesenchymal stem cells; signal transducer and activator of transcription 3; transformation growth factor-β
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Year: 2013 PMID： 24145153 DOI： 10.1016/j.canlet.2013.09.017
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679