Literature DB >> 24144410

Selection and optimization of hits from a high-throughput phenotypic screen against Trypanosoma cruzi.

Martine Keenan1, Paul W Alexander, Jason H Chaplin, Michael J Abbott, Hugo Diao, Zhisen Wang, Wayne M Best, Catherine J Perez, Scott M J Cornwall, Sarah K Keatley, R C Andrew Thompson, Susan A Charman, Karen L White, Eileen Ryan, Gong Chen, Jean-Robert Ioset, Thomas W von Geldern, Eric Chatelain.   

Abstract

BACKGROUND: Inhibitors of Trypanosoma cruzi with novel mechanisms of action are urgently required to diversify the current clinical and preclinical pipelines. Increasing the number and diversity of hits available for assessment at the beginning of the discovery process will help to achieve this aim.
RESULTS: We report the evaluation of multiple hits generated from a high-throughput screen to identify inhibitors of T. cruzi and from these studies the discovery of two novel series currently in lead optimization. Lead compounds from these series potently and selectively inhibit growth of T. cruzi in vitro and the most advanced compound is orally active in a subchronic mouse model of T. cruzi infection.
CONCLUSION: High-throughput screening of novel compound collections has an important role to play in diversifying the trypanosomatid drug discovery portfolio. A new T. cruzi inhibitor series with good drug-like properties and promising in vivo efficacy has been identified through this process.

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Year:  2013        PMID: 24144410     DOI: 10.4155/fmc.13.139

Source DB:  PubMed          Journal:  Future Med Chem        ISSN: 1756-8919            Impact factor:   3.808


  12 in total

1.  A new chemotype with promise against Trypanosoma cruzi.

Authors:  Xiaofang Wang; Monica Cal; Marcel Kaiser; Frederick S Buckner; Galina I Lepesheva; Austin G Sanford; Alexander I Wallick; Paul H Davis; Jonathan L Vennerstrom
Journal:  Bioorg Med Chem Lett       Date:  2019-10-31       Impact factor: 2.823

Review 2.  Hit and lead criteria in drug discovery for infectious diseases of the developing world.

Authors:  Kei Katsuno; Jeremy N Burrows; Ken Duncan; Rob Hooft van Huijsduijnen; Takushi Kaneko; Kiyoshi Kita; Charles E Mowbray; Dennis Schmatz; Peter Warner; B T Slingsby
Journal:  Nat Rev Drug Discov       Date:  2015-10-05       Impact factor: 84.694

3.  Miltefosine and Benznidazole Combination Improve Anti-Trypanosoma cruzi In Vitro and In Vivo Efficacy.

Authors:  Julián Ernesto Nicolás Gulin; Margarita María Catalina Bisio; Daniela Rocco; Jaime Altcheh; María Elisa Solana; Facundo García-Bournissen
Journal:  Front Cell Infect Microbiol       Date:  2022-07-05       Impact factor: 6.073

Review 4.  Drug strategies targeting CYP51 in neglected tropical diseases.

Authors:  Jun Yong Choi; Larissa M Podust; William R Roush
Journal:  Chem Rev       Date:  2014-10-22       Impact factor: 60.622

5.  Identification of Trypanocidal Activity for Known Clinical Compounds Using a New Trypanosoma cruzi Hit-Discovery Screening Cascade.

Authors:  Manu De Rycker; John Thomas; Jennifer Riley; Stephen J Brough; Tim J Miles; David W Gray
Journal:  PLoS Negl Trop Dis       Date:  2016-04-15

6.  In vitro drug susceptibility of two strains of the wildlife trypanosome, Trypanosoma copemani: A comparison with Trypanosoma cruzi.

Authors:  Adriana Botero; Sarah Keatley; Christopher Peacock; R C Andrew Thompson
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2016-12-23       Impact factor: 4.077

7.  Discovery and Optimization of 5-Amino-1,2,3-triazole-4-carboxamide Series against Trypanosoma cruzi.

Authors:  Stephen Brand; Eun Jung Ko; Elisabet Viayna; Stephen Thompson; Daniel Spinks; Michael Thomas; Lars Sandberg; Amanda F Francisco; Shiromani Jayawardhana; Victoria C Smith; Chimed Jansen; Manu De Rycker; John Thomas; Lorna MacLean; Maria Osuna-Cabello; Jennifer Riley; Paul Scullion; Laste Stojanovski; Frederick R C Simeons; Ola Epemolu; Yoko Shishikura; Sabrinia D Crouch; Tania S Bakshi; Christopher J Nixon; Iain H Reid; Alan P Hill; Tim Z Underwood; Sean J Hindley; Sharon A Robinson; John M Kelly; Jose M Fiandor; Paul G Wyatt; Maria Marco; Timothy J Miles; Kevin D Read; Ian H Gilbert
Journal:  J Med Chem       Date:  2017-08-27       Impact factor: 7.446

Review 8.  Kinases as druggable targets in trypanosomatid protozoan parasites.

Authors:  Christopher Merritt; Lisseth E Silva; Angela L Tanner; Kenneth Stuart; Michael P Pollastri
Journal:  Chem Rev       Date:  2014-10-07       Impact factor: 60.622

9.  3-pyridyl inhibitors with novel activity against Trypanosoma cruzi reveal in vitro profiles can aid prediction of putative cytochrome P450 inhibition.

Authors:  Melissa L Sykes; Vicky M Avery
Journal:  Sci Rep       Date:  2018-03-20       Impact factor: 4.379

10.  Novel and Selective Rhipicephalus microplus Triosephosphate Isomerase Inhibitors with Acaricidal Activity.

Authors:  Luiz Saramago; Helga Gomes; Elena Aguilera; Hugo Cerecetto; Mercedes González; Mauricio Cabrera; Maria Fernanda Alzugaray; Itabajara da Silva Vaz Junior; Rodrigo Nunes da Fonseca; Beatriz Aguirre-López; Nallely Cabrera; Ruy Pérez-Montfort; Alicia Merlino; Jorge Moraes; Guzmán Álvarez
Journal:  Vet Sci       Date:  2018-08-23
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