Ali Reza Chavshin1, Mohammad Ali Oshaghi, Hasan Vatandoost, Ahmad Ali Hanafi-Bojd, Ahmad Raeisi, Fatemeh Nikpoor. 1. Social Determinants of Health Research Center, Urmia University of Medical Sciences, Urmia, Iran; Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences (UMSU), Urmia, Iran; Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Abstract
OBJECTIVE: To identify the biological forms, sporozoite rate and molecular characterization of the Anopheles stephensi (An. stephensi) in Hormozgan and Sistan-Baluchistan provinces, the most important malarious areas in Iran. METHODS: Wild live An. stephensi samples were collected from different malarious areas in southern Iran. The biological forms were identified based on number of egg-ridges. Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II (mtDNA-COI/COII). The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method. RESULTS: Results showed that all three biological forms including mysorensis, intermediate and type are present in the study areas. Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples. CONCLUSIONS: Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations. Lack of malaria parasite infection in An. stephensi, the most important vector of malaria, may be partly due to the success and achievement of ongoing active malaria control program in the region.
OBJECTIVE: To identify the biological forms, sporozoite rate and molecular characterization of the Anopheles stephensi (An. stephensi) in Hormozgan and Sistan-Baluchistan provinces, the most important malarious areas in Iran. METHODS: Wild live An. stephensi samples were collected from different malarious areas in southern Iran. The biological forms were identified based on number of egg-ridges. Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II (mtDNA-COI/COII). The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method. RESULTS: Results showed that all three biological forms including mysorensis, intermediate and type are present in the study areas. Molecular investigations revealed no genetic variation between mtDNA COI/COII sequences of the biological forms and no Plasmodium parasites was detected in the collected mosquito samples. CONCLUSIONS: Presence of three biological forms with identical sequences showed that the known biological forms belong to a single taxon and the various vectorial capacities reported for these forms are more likely corresponded to other epidemiological factors than to the morphotype of the populations. Lack of malaria parasite infection in An. stephensi, the most important vector of malaria, may be partly due to the success and achievement of ongoing active malaria control program in the region.
Authors: Ali Reza Chavshin; Mohammad Ali Oshaghi; Hasan Vatandoost; Mohammad Reza Pourmand; Ahmad Raeisi; Ahmad Ali Enayati; Nadia Mardani; Sadigheh Ghoorchian Journal: Acta Trop Date: 2011-11-04 Impact factor: 3.112
Authors: Ahmad Mehravaran; Hassan Vatandoost; Mohammad Ali Oshaghi; Mohammad Reza Abai; Hamideh Edalat; Ezatoddin Javadian; Minoo Mashayekhi; Norair Piazak; Ahmad Ali Hanafi-Bojd Journal: Acta Med Iran Date: 2012
Authors: A A Hanafi-Bojd; H Vatandoost; M A Oshaghi; A A Haghdoost; M Shahi; M M Sedaghat; F Abedi; M Yeryan; A Pakari Journal: Acta Trop Date: 2011-05-06 Impact factor: 3.112