Gao-Feng Xu1, Wan-Fu Xie. 1. Department of Neurosurgery, First Hospital of Xi'an, Jiaotong University, Xi'an 710061, China.
Abstract
OBJECTIVE: To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells. METHODS: Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot. RESULTS: ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions. CONCLUSIONS: ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
OBJECTIVE: To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells. METHODS:Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot. RESULTS:ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions. CONCLUSIONS:ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
Authors: József Virga; László Bognár; Tibor Hortobágyi; Gábor Zahuczky; Éva Csősz; Gergő Kalló; Judit Tóth; Gábor Hutóczki; Judit Reményi-Puskár; László Steiner; Almos Klekner Journal: Pathol Oncol Res Date: 2017-02-04 Impact factor: 3.201