Lin Tan1, Jin-Tai Yu2, Qiu-Yan Liu1, Meng-Shan Tan3, Wei Zhang1, Nan Hu1, Ying-Li Wang1, Lei Sun1, Teng Jiang4, Lan Tan5. 1. Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, PR China. 2. Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing, PR China. Electronic address: yu-jintai@163.com. 3. Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Qingdao, PR China. 4. Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing, PR China. 5. Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, College of Medicine and Pharmaceutics, Ocean University of China, Qingdao, PR China; Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing, PR China. Electronic address: dr.tanlan@163.com.
Abstract
BACKGROUND: MicroRNAs (miRNAs) are endogenous small RNAs of 21-25 nucleotides that post-transcriptionally regulate gene expressions. Recently, circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system. This study was conducted to investigate the potential role of serum miRNAs as diagnostic biomarkers for Alzheimer's disease (AD). METHODS: Serum samples were obtained from 105 probable AD patients and 150 age- and gender-matched normal controls. The serum concentrations of miRNAs miR-9, miR-29a, miR-29b, miR-101, miR-125b, and miR-181c were measured with a real-time quantitative reverse transcriptase PCR (qRT-PCR) method. RESULTS: We found both miR-125b and miR-181c were down-regulated while miR-9 was up-regulated in serum of AD patients compared with that of normal controls. Among the receiver operating characteristic (ROC) results, miR-125b alone showed its priority with a specificity up to 68.3% and a sensitivity of 80.8%. Importantly, miR-125b was correlated with the Mini Mental State Examination (MMSE) in AD patients. CONCLUSIONS: Our results indicate that serum miR-125b may serve as a useful noninvasive biomarker for AD.
BACKGROUND: MicroRNAs (miRNAs) are endogenous small RNAs of 21-25 nucleotides that post-transcriptionally regulate gene expressions. Recently, circulating miRNAs have been reported as promising biomarkers for neurodegenerative disorders and processes affecting the central nervous system. This study was conducted to investigate the potential role of serum miRNAs as diagnostic biomarkers for Alzheimer's disease (AD). METHODS: Serum samples were obtained from 105 probable ADpatients and 150 age- and gender-matched normal controls. The serum concentrations of miRNAs miR-9, miR-29a, miR-29b, miR-101, miR-125b, and miR-181c were measured with a real-time quantitative reverse transcriptase PCR (qRT-PCR) method. RESULTS: We found both miR-125b and miR-181c were down-regulated while miR-9 was up-regulated in serum of ADpatients compared with that of normal controls. Among the receiver operating characteristic (ROC) results, miR-125b alone showed its priority with a specificity up to 68.3% and a sensitivity of 80.8%. Importantly, miR-125b was correlated with the Mini Mental State Examination (MMSE) in ADpatients. CONCLUSIONS: Our results indicate that serum miR-125b may serve as a useful noninvasive biomarker for AD.
Authors: Marta Cosín-Tomás; Anna Antonell; Albert Lladó; Daniel Alcolea; Juan Fortea; Mario Ezquerra; Albert Lleó; Maria José Martí; Mercè Pallàs; Raquel Sanchez-Valle; José Luís Molinuevo; Coral Sanfeliu; Perla Kaliman Journal: Mol Neurobiol Date: 2016-09-08 Impact factor: 5.590
Authors: Adrià Dangla-Valls; José Luis Molinuevo; Jordi Altirriba; Raquel Sánchez-Valle; Daniel Alcolea; Juan Fortea; Lorena Rami; Mircea Balasa; Cristina Muñoz-García; Mario Ezquerra; Rubén Fernández-Santiago; Alberto Lleó; Albert Lladó; Anna Antonell Journal: Mol Neurobiol Date: 2016-10-13 Impact factor: 5.590