BACKGROUND: Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. Some hepcidin truncated peptides have antibiotic effects. RESULTS: A new analytical method for hepcidin determination in human plasma using LC-HRMS operating in full-scan acquisition mode has been validated. The extraction consists of protein precipitation and a drying reconstitution step; a 2.1 x 50 mm (idxL) C18 analytical column was used. Detection specificity, stability, accuracy, precision and recoveries were determined. The LOQ/LOD were 0.25/0.1 nM, respectively. More than 600 injections of plasma extracts were performed, allowing evaluation of the assay robustness. Hepcidin-20, hepcidin-22 and a new isoform, hepcidin-24, were detected in patients. CONCLUSION: The data underscore the usefulness of LC-HRMS for in-depth investigations related to hepcidin levels and pathways.
BACKGROUND:Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. Some hepcidin truncated peptides have antibiotic effects. RESULTS: A new analytical method for hepcidin determination in human plasma using LC-HRMS operating in full-scan acquisition mode has been validated. The extraction consists of protein precipitation and a drying reconstitution step; a 2.1 x 50 mm (idxL) C18 analytical column was used. Detection specificity, stability, accuracy, precision and recoveries were determined. The LOQ/LOD were 0.25/0.1 nM, respectively. More than 600 injections of plasma extracts were performed, allowing evaluation of the assay robustness. Hepcidin-20, hepcidin-22 and a new isoform, hepcidin-24, were detected in patients. CONCLUSION: The data underscore the usefulness of LC-HRMS for in-depth investigations related to hepcidin levels and pathways.
Authors: Susan E Abbatiello; Birgit Schilling; D R Mani; Lisa J Zimmerman; Steven C Hall; Brendan MacLean; Matthew Albertolle; Simon Allen; Michael Burgess; Michael P Cusack; Mousumi Gosh; Victoria Hedrick; Jason M Held; H Dorota Inerowicz; Angela Jackson; Hasmik Keshishian; Christopher R Kinsinger; John Lyssand; Lee Makowski; Mehdi Mesri; Henry Rodriguez; Paul Rudnick; Pawel Sadowski; Nell Sedransk; Kent Shaddox; Stephen J Skates; Eric Kuhn; Derek Smith; Jeffery R Whiteaker; Corbin Whitwell; Shucha Zhang; Christoph H Borchers; Susan J Fisher; Bradford W Gibson; Daniel C Liebler; Michael J MacCoss; Thomas A Neubert; Amanda G Paulovich; Fred E Regnier; Paul Tempst; Steven A Carr Journal: Mol Cell Proteomics Date: 2015-02-18 Impact factor: 5.911
Authors: Monique P Gelderman; Jin Hyen Baek; Ayla Yalamanoglu; Michele Puglia; Florence Vallelian; Bo Burla; Jaroslav Vostal; Dominik J Schaer; Paul W Buehler Journal: Haematologica Date: 2015-01-23 Impact factor: 9.941
Authors: Sophie Waldvogel-Abramowski; Gérard Waeber; Christoph Gassner; Andreas Buser; Beat M Frey; Bernard Favrat; Jean-Daniel Tissot Journal: Transfus Med Hemother Date: 2014-05-12 Impact factor: 3.747
Authors: Steven A Carr; Susan E Abbatiello; Bradley L Ackermann; Christoph Borchers; Bruno Domon; Eric W Deutsch; Russell P Grant; Andrew N Hoofnagle; Ruth Hüttenhain; John M Koomen; Daniel C Liebler; Tao Liu; Brendan MacLean; D R Mani; Elizabeth Mansfield; Hendrik Neubert; Amanda G Paulovich; Lukas Reiter; Olga Vitek; Ruedi Aebersold; Leigh Anderson; Robert Bethem; Josip Blonder; Emily Boja; Julianne Botelho; Michael Boyne; Ralph A Bradshaw; Alma L Burlingame; Daniel Chan; Hasmik Keshishian; Eric Kuhn; Christopher Kinsinger; Jerry S H Lee; Sang-Won Lee; Robert Moritz; Juan Oses-Prieto; Nader Rifai; James Ritchie; Henry Rodriguez; Pothur R Srinivas; R Reid Townsend; Jennifer Van Eyk; Gordon Whiteley; Arun Wiita; Susan Weintraub Journal: Mol Cell Proteomics Date: 2014-01-17 Impact factor: 5.911