AIM: To analyze the genotoxic effect of two hydrogen peroxide-containing bleaching products on oral mucosal cells. MATERIALS AND METHODS: The research was conducted on 22 individuals divided into two groups. Group 1 used ZOOM2 and group 2 the Opalescence BOOST bleaching agent. Specimens of the gingival and the upper lip mucosa were obtained before, immediately after, and 72 h after the bleaching procedure and were analyzed using a micronucleus test. RESULTS: Seventy-two hours after bleaching treatment with BOOST, samples collected from the oral mucosa exhibited a statistically significant increase of all genotoxicity markers, with large effect sizes (Cohen's d>0.8) observed in the total number of micronuclei (MN), number of cells with 3+ MN, karyolysis and bi-nuclear cells. ZOOM2 treatment showed a significant increase, with medium-to-large effect sizes, in the number of cells with 1 MN, karyolysis, nuclear buds and bi-nuclear cells. CONCLUSION: Both preparations demonstrated potential genotoxic effects.
RCT Entities:
AIM: To analyze the genotoxic effect of two hydrogen peroxide-containing bleaching products on oral mucosal cells. MATERIALS AND METHODS: The research was conducted on 22 individuals divided into two groups. Group 1 used ZOOM2 and group 2 the Opalescence BOOST bleaching agent. Specimens of the gingival and the upper lip mucosa were obtained before, immediately after, and 72 h after the bleaching procedure and were analyzed using a micronucleus test. RESULTS: Seventy-two hours after bleaching treatment with BOOST, samples collected from the oral mucosa exhibited a statistically significant increase of all genotoxicity markers, with large effect sizes (Cohen's d>0.8) observed in the total number of micronuclei (MN), number of cells with 3+ MN, karyolysis and bi-nuclear cells. ZOOM2 treatment showed a significant increase, with medium-to-large effect sizes, in the number of cells with 1 MN, karyolysis, nuclear buds and bi-nuclear cells. CONCLUSION: Both preparations demonstrated potential genotoxic effects.
Authors: Marcelino Benvindo-Souza; Rhayane Alves Assis; Eliane Andreia Santos Oliveira; Rinneu Elias Borges; Lia Raquel de Souza Santos Journal: Environ Sci Pollut Res Int Date: 2017-11-19 Impact factor: 4.223
Authors: Valderlane L P Colares; Suellen N L Lima; Nágila C F Sousa; Mizael C Araújo; Domingos M S Pereira; Saulo J F Mendes; Simone A Teixeira; Cristina de A Monteiro; Matheus C Bandeca; Walter L Siqueira; Eduardo B Moffa; Marcelo N Muscará; Elizabeth S Fernandes Journal: Sci Rep Date: 2019-03-05 Impact factor: 4.379