BACKGROUND: Three functionally distinct monocyte subsets have been identified. Statins are of undoubted effect in atherosclerosis and have numerous pleiotropic effects that contribute to their clinical success, but the effect of these drugs on monocyte subsets is unclear. We hypothesised a beneficial effect of statins on key receptor expression by monocyte subsets. MATERIAL AND METHODS: Effects of temporal (2 weeks) cessation of statin therapy by 66 patients with stable coronary artery disease on monocyte subsets [CD14++CD16-CCR2+ (Mon1), CD14++CD16+CCR2+ (Mon2) and CD14+CD16++CCR2- (Mon3)], their aggregates with platelets and their expression of a number of receptors involved in inflammation (IL-6 receptor), adhesion [vascular cell adhesion molecule (VCAM)], angiogenesis [vascular endothelial growth factor (VEGF)] and repair were assessed by flow cytometry. RESULTS: Statin cessation did not lead to any significant changes in absolute numbers of monocyte subsets or the degree of their aggregation with platelets. All monocyte subsets showed significant downregulation of expression of vascular endothelial factor receptor 2, Tie2 and Toll-like receptor-4 (TLR4; all changes P < 0·01). Expression of CXCR4 was only reduced in Mon1 cells (P = 0·013). There was no significant change in the expression of CD14, CD16, CCR4, IL6 receptor and VCAM (all P = NS). CONCLUSIONS: Statin withdrawal does not affect counts of any of monocyte subsets, but leads to downregulation of expression of TLR4 and receptors related to angiogenesis on all subsets, as well as a decrease in density of CXCR4 expression on 'classical' Mon1. These data provide further support of pleiotropic effects of statins and their effects on monocyte pro-angiogenic and proreparative characteristics.
BACKGROUND: Three functionally distinct monocyte subsets have been identified. Statins are of undoubted effect in atherosclerosis and have numerous pleiotropic effects that contribute to their clinical success, but the effect of these drugs on monocyte subsets is unclear. We hypothesised a beneficial effect of statins on key receptor expression by monocyte subsets. MATERIAL AND METHODS: Effects of temporal (2 weeks) cessation of statin therapy by 66 patients with stable coronary artery disease on monocyte subsets [CD14++CD16-CCR2+ (Mon1), CD14++CD16+CCR2+ (Mon2) and CD14+CD16++CCR2- (Mon3)], their aggregates with platelets and their expression of a number of receptors involved in inflammation (IL-6 receptor), adhesion [vascular cell adhesion molecule (VCAM)], angiogenesis [vascular endothelial growth factor (VEGF)] and repair were assessed by flow cytometry. RESULTS: Statin cessation did not lead to any significant changes in absolute numbers of monocyte subsets or the degree of their aggregation with platelets. All monocyte subsets showed significant downregulation of expression of vascular endothelial factor receptor 2, Tie2 and Toll-like receptor-4 (TLR4; all changes P < 0·01). Expression of CXCR4 was only reduced in Mon1 cells (P = 0·013). There was no significant change in the expression of CD14, CD16, CCR4, IL6 receptor and VCAM (all P = NS). CONCLUSIONS: Statin withdrawal does not affect counts of any of monocyte subsets, but leads to downregulation of expression of TLR4 and receptors related to angiogenesis on all subsets, as well as a decrease in density of CXCR4 expression on 'classical' Mon1. These data provide further support of pleiotropic effects of statins and their effects on monocyte pro-angiogenic and proreparative characteristics.
Authors: Ching-Jen Chen; Dale Ding; Natasha Ironside; Thomas J Buell; Lori J Elder; Amy Warren; Amy P Adams; Sarah J Ratcliffe; Robert F James; Neeraj S Naval; Bradford B Worrall; Karen C Johnston; Andrew M Southerland Journal: Neurology Date: 2019-11-11 Impact factor: 9.910
Authors: Konstantin A Krychtiuk; Stefan P Kastl; Stefan Pfaffenberger; Max Lenz; Sebastian L Hofbauer; Anna Wonnerth; Lorenz Koller; Katharina M Katsaros; Thomas Pongratz; Georg Goliasch; Alexander Niessner; Ludovit Gaspar; Kurt Huber; Gerald Maurer; Elisabeth Dostal; Johann Wojta; Stanislav Oravec; Walter S Speidl Journal: PLoS One Date: 2015-04-07 Impact factor: 3.240
Authors: Xiang Li; Daniel Heber; Tatjana Leike; Dietrich Beitzke; Xia Lu; Xiaoli Zhang; Yongxiang Wei; Markus Mitterhauser; Wolfgang Wadsak; Saskia Kropf; Hans J Wester; Christian Loewe; Marcus Hacker; Alexander R Haug Journal: Eur J Nucl Med Mol Imaging Date: 2017-09-21 Impact factor: 9.236
Authors: Konstantin A Krychtiuk; Stefan P Kastl; Sebastian L Hofbauer; Anna Wonnerth; Georg Goliasch; Maria Ozsvar-Kozma; Katharina M Katsaros; Gerald Maurer; Kurt Huber; Elisabeth Dostal; Christoph J Binder; Stefan Pfaffenberger; Stanislav Oravec; Johann Wojta; Walter S Speidl Journal: J Clin Lipidol Date: 2015-04-25 Impact factor: 4.766