Arthrobacter gyeryongensis sp. nov., isolated from soil of a Gynostemma pentaphyllum field.

A Gram-stain-positive, flagellate, rod-shaped, catalase- and oxidase-positive bacterium, designated DCY72(T), was isolated from the soil of a Gynostemma pentaphyllum field. Growth occurred at 4-34 °C (optimum 30 °C), at pH 4-10 (optimum pH 7), and with 0-5% NaCl (w/v). The major menaquinones of strain DCY72(T) were MK-9(H2) (81.0 %) and MK-10(H2) (12.2%). The major amino acid present in the cell-wall peptidoglycan was L-lysine. The major fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0). The genomic DNA G+C content was 64.5 mol%. 16S rRNA gene sequence analysis revealed that strain DCY72(T) belonged to the family Micrococcaceae and was most closely related to Arthrobacter ramosus CCM 1646(T) (98.2% similarity). The DNA-DNA relatedness between strain DCY72(T) and A. ramosus KACC 14391(T) (98.2% 16S rRNA gene sequence similarity), Arthrobacter nitroguajacolicus KACC 14581(T) (97.6%), Arthrobacter nicotinovorans KACC 20508(T) (97.3%) and Arthrobacter aurescens KACC 20528(T) (97.3%). was 12.9% ± 0.3, 25.6% ± 0.3, 26.6% ± 0.5 and 23.2% ± 0.9, respectively. On the basis of the phenotypic characteristics, genotypic analysis and physiological characteristics, strain DCY72(T) represents a novel species of the genus Arthrobacter, for which the name Arthrobacter gyeryongensis sp. nov. is proposed. The type strain is DCY72(T) ( = KCTC 33072(T) = JCM 18514(T)).