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Literature DB >> 241328

Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos.

Abstract

A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion of sodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximum in 2 1/2 min, well ahead of polyribosome peptides which, as described by Herrmann et al. (1971), attain maximum specific radioactivity in about 10 min.

Entities: Chemical Species

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Year: 1975 PMID： 241328 PMCID： PMC1165474 DOI： 10.1042/bj1470473

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. THE NUMBER OF SOLUBLE RNA MOLECULES ON RETICULOCYTE POLYRIBOSOMES.

Authors: J R WARNER; A RICH
Journal: Proc Natl Acad Sci U S A Date: 1964-06 Impact factor: 11.205

2. BINDING OF TRANSFER RIBONUCLEIC ACID TO RIBOSOMES ENGAGED IN PROTEIN SYNTHESIS: NUMBER AND PROPERTIES OF RIBOSOMAL BINDING SITES.

Authors: F O WETTSTEIN; H NOLL
Journal: J Mol Biol Date: 1965-01 Impact factor: 5.469

7. Studies of muscle development. VII. Transfer of amino acids from blood plasma to the cell pool and polysome peptides in muscle of the chick embryo.

Authors: H Herrmann; S M Heywood; M Nwagwu
Journal: Exp Cell Res Date: 1971-12 Impact factor: 3.905

8. Complex of aminoacyl-transfer RNA synthetases.

Authors: A K Bandyopadhyay; M P Deutscher
Journal: J Mol Biol Date: 1971-08-28 Impact factor: 5.469

9. Distribution of 5 s RNA in HeLa cells.

Authors: E Knight; J E Darnell
Journal: J Mol Biol Date: 1967-09-28 Impact factor: 5.469

10. Relationship between intracellular amino acids and protein synthesis in the extensor digitorum longus muscle of rats.

Authors: R C Hider; E B Fern; D R London
Journal: Biochem J Date: 1969-09 Impact factor: 3.857

1 in total

1. Kinetics of initiating polypeptide elongation in an IRES-dependent system.

Authors: Haibo Zhang; Martin Y Ng; Yuanwei Chen; Barry S Cooperman
Journal: Elife Date: 2016-06-02 Impact factor: 8.140

1 in total

Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos.

1. THE NUMBER OF SOLUBLE RNA MOLECULES ON RETICULOCYTE POLYRIBOSOMES.

2. BINDING OF TRANSFER RIBONUCLEIC ACID TO RIBOSOMES ENGAGED IN PROTEIN SYNTHESIS: NUMBER AND PROPERTIES OF RIBOSOMAL BINDING SITES.

3. Transfer RNA content of runoff and complexed ribosomes of Escherichia coli.

4. Compartmentation of free amino acids for protein synthesis in rat liver.

5. Quantitative measurement of active polysomes of developing chick muscle.

6. Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis.

7. Studies of muscle development. VII. Transfer of amino acids from blood plasma to the cell pool and polysome peptides in muscle of the chick embryo.

8. Complex of aminoacyl-transfer RNA synthetases.

9. Distribution of 5 s RNA in HeLa cells.

10. Relationship between intracellular amino acids and protein synthesis in the extensor digitorum longus muscle of rats.

1. Kinetics of initiating polypeptide elongation in an IRES-dependent system.