Literature DB >> 24132604

Antigenicity of recombinant maltose binding protein-Mycobacterium avium subsp. paratuberculosis fusion proteins with and without factor Xa cleaving.

Ratna B Gurung1, Douglas J Begg, Auriol C Purdie, John P Bannantine, Richard J Whittington.   

Abstract

Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants. Proteomic studies have shown that M. avium subsp. paratuberculosis expresses certain proteins when exposed to in vitro physiological stress conditions similar to the conditions experienced within a host during natural infection. Such proteins are hypothesized to be expressed in vivo, are recognized by the host immune system, and may be of potential use in the diagnosis of JD. In this study, 50 recombinant maltose binding protein (MBP)-M. avium subsp. paratuberculosis fusion proteins were evaluated using serum samples from sheep infected with M. avium subsp. paratuberculosis, and 29 (58%) were found to be antigenic. Among 50 fusion proteins, 10 were evaluated in MBP fusion and factor Xa-cleaved forms. A total of 31 proteins (62%) were found to be antigenic in either MBP fusion or factor Xa-cleaved forms. Antigenicity after cleavage and removal of the MBP tag was marginally enhanced.

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Year:  2013        PMID： 24132604      PMCID： PMC3889514          DOI： 10.1128/CVI.00596-13

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN： 1556-679X


  47 in total

1.  Bovine NK cells can produce gamma interferon in response to the secreted mycobacterial proteins ESAT-6 and MPP14 but not in response to MPB70.

Authors:  Ingrid Olsen; Preben Boysen; Siri Kulberg; Jayne C Hope; Gregers Jungersen; Anne K Storset
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

2.  MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease.

Authors:  John P Bannantine; Cari K Lingle; Judith R Stabel; Kasra X Ramyar; Brandon L Garcia; Alex J Raeber; Pascal Schacher; Vivek Kapur; Brian V Geisbrecht
Journal:  Clin Vaccine Immunol       Date:  2012-05-16

3.  Identification and characterization of a gene encoding a 35-kDa protein from Mycobacterium avium subspecies paratuberculosis.

Authors:  K D Banasure; S H Basagoudanavar; P Chaudhury; V Tiwari; N S Parihar; P P Goswami
Journal:  FEMS Microbiol Lett       Date:  2001-03-15       Impact factor: 2.742

4.  Galectin-1 as a fusion partner for the production of soluble and folded human beta-1,4-galactosyltransferase-T7 in E. coli.

Authors:  Marta Pasek; Elizabeth Boeggeman; Boopathy Ramakrishnan; Pradman K Qasba
Journal:  Biochem Biophys Res Commun       Date:  2010-03-11       Impact factor: 3.575

5.  Immunogenicity and reactivity of novel Mycobacterium avium subsp. paratuberculosis PPE MAP1152 and conserved MAP1156 proteins with sera from experimentally and naturally infected animals.

Authors:  John P Bannantine; Avery L Paulson; Ofelia Chacon; Robert J Fenton; Denise K Zinniel; David S McVey; David R Smith; Charles J Czuprynski; Raúl G Barletta
Journal:  Clin Vaccine Immunol       Date:  2010-11-17

Review 6.  Reactive nitrogen and oxygen intermediates and bacterial defenses: unusual adaptations in Mycobacterium tuberculosis.

Authors:  Thomas C Zahrt; Vojo Deretic
Journal:  Antioxid Redox Signal       Date:  2002-02       Impact factor: 8.401

7.  Immunogenicity of proteome-determined Mycobacterium avium subsp. paratuberculosis-specific proteins in sheep with paratuberculosis.

Authors:  Valerie Hughes; John P Bannantine; Susan Denham; Stuart Smith; Alfredo Garcia-Sanchez; Jill Sales; Michael L Paustian; Kevin Mclean; Karen Stevenson
Journal:  Clin Vaccine Immunol       Date:  2008-10-08

8.  Experimental infection model for Johne's disease using a lyophilised, pure culture, seedstock of Mycobacterium avium subspecies paratuberculosis.

Authors:  Douglas J Begg; Kumudika de Silva; Lyrissa Di Fiore; Deborah L Taylor; Katrina Bower; Ling Zhong; Satoko Kawaji; David Emery; Richard J Whittington
Journal:  Vet Microbiol       Date:  2009-09-11       Impact factor: 3.293

9.  Survival and dormancy of Mycobacterium avium subsp. paratuberculosis in the environment.

Authors:  Richard J Whittington; D Jeff Marshall; Paul J Nicholls; Ian B Marsh; Leslie A Reddacliff
Journal:  Appl Environ Microbiol       Date:  2004-05       Impact factor: 4.792

10.  In silico screened Mycobacterium avium subsp. paratuberculosis (MAP) recombinant proteins upregulated under stress conditions are immunogenic in sheep.

Authors:  Ratna B Gurung; Auriol C Purdie; Douglas J Begg; Richard J Whittington
Journal:  Vet Immunol Immunopathol       Date:  2012-07-07       Impact factor: 2.046
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  3 in total

1.  Identification of Novel Seroreactive Antigens in Johne's Disease Cattle by Using the Mycobacterium tuberculosis Protein Array.

Authors:  John P Bannantine; Joseph J Campo; Lingling Li; Arlo Randall; Jozelyn Pablo; Craig A Praul; Juan Antonio Raygoza Garay; Judith R Stabel; Vivek Kapur
Journal:  Clin Vaccine Immunol       Date:  2017-07-05

2.  Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays.

Authors:  Lingling Li; Bettina Wagner; Heather Freer; Megan Schilling; John P Bannantine; Joseph J Campo; Robab Katani; Yrjo T Grohn; Jessica Radzio-Basu; Vivek Kapur
Journal:  PLoS One       Date:  2017-12-19       Impact factor: 3.240

3.  Identification of sero-reactive antigens for the early diagnosis of Johne's disease in cattle.

Authors:  Lingling Li; John P Bannantine; Joseph J Campo; Arlo Randall; Yrjo T Grohn; Robab Katani; Megan Schilling; Jessica Radzio-Basu; Vivek Kapur
Journal:  PLoS One       Date:  2017-09-01       Impact factor: 3.240
  3 in total

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