Literature DB >> 2413128

An improved method of antigen detection on nitrocellulose: in situ staining of alkaline phosphatase conjugated antibody.

M L Dao.   

Abstract

A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.

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Year:  1985        PMID： 2413128     DOI： 10.1016/0022-1759(85)90354-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  18 in total

1.  T and B cell reactivity to adrenal antigens in autoimmune Addison's disease.

Authors:  M Freeman; A P Weetman
Journal:  Clin Exp Immunol       Date:  1992-05       Impact factor: 4.330

2.  A nitrocellulose membrane-based ELISA for the detection of Plasmodium infections in mosquitos.

Authors:  B L Petros; P M Procell; G H Campbell; F H Collins
Journal:  Bull World Health Organ       Date:  1989       Impact factor: 9.408

3.  Species variation in transcription factor IIIA.

Authors:  C J Gaskins; R M Fiser-Littell; A L Duke; J S Hanas
Journal:  Nucleic Acids Res       Date:  1989-01-25       Impact factor: 16.971

4.  Immunologic cross-reactivity of type A streptococcal exotoxin (erythrogenic toxin) and staphylococcal enterotoxins B and C1.

Authors:  W L Hynes; C R Weeks; J J Iandolo; J J Ferretti
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441

5.  Molecular cloning of the Streptococcus mutans gene specifying antigen A.

Authors:  M L Dao; C Chavez; Y Hirachi; J J Ferretti
Journal:  Infect Immun       Date:  1989-11       Impact factor: 3.441

6.  Cell-associated collagenolytic activity by group B streptococci.

Authors:  R J Jackson; M L Dao; D V Lim
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

7.  Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes.

Authors:  A Hemphill; B Gottstein
Journal:  Parasitol Res       Date:  1995       Impact factor: 2.289

8.  Bacteremia due to Rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory.

Authors:  D F Welch; D M Hensel; D A Pickett; V H San Joaquin; A Robinson; L N Slater
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

9.  Inactivation of the Streptococcus mutans wall-associated protein A gene (wapA) results in a decrease in sucrose-dependent adherence and aggregation.

Authors:  H Qian; M L Dao
Journal:  Infect Immun       Date:  1993-12       Impact factor: 3.441

10.  Implications of anti-Ro/Sjögren's syndrome A antigen autoantibody in normal sera for autoimmunity.

Authors:  K K Gaither; O F Fox; H Yamagata; M J Mamula; M Reichlin; J B Harley
Journal:  J Clin Invest       Date:  1987-03       Impact factor: 14.808
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