Literature DB >> 2413068

Detection of Escherichia coli adhesins with DNA probes.

J A Lanser, P A Anargyros.   

Abstract

DNA hybridization assays for genes encoding the Escherichia coli adhesins K88 and K99 were developed. These assays were used to screen a variety of animal E. coli strains, and the results were compared with results obtained by serological methods. All methods compared well for determining the presence of adhesin K99. However, the DNA hybridization detected more K88-positive strains than did either enzyme immunoassay or slide agglutination. Agreement was improved when strains were grown on blood or nutrient agar instead of Minca medium.

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Year:  1985        PMID： 2413068      PMCID： PMC268425          DOI： 10.1128/jcm.22.3.425-427.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Simultaneous occurrence of E. coli B and Lantigens in strains from diseased swine. Influence of cultivation temperature. Two new E. coli Kantigens: K 87 and K 88.

Authors:  I ORSKOV; F ORSKOV; W J SOJKA; J M LEACH
Journal:  Acta Pathol Microbiol Scand       Date:  1961

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli.

Authors:  P A Guinée; J Veldkamp; W H Jansen
Journal:  Infect Immun       Date:  1977-02       Impact factor: 3.441

4.  Organization and expression of genes involved in the biosynthesis of K99 fimbriae.

Authors:  F K de Graaf; B E Krenn; P Klaasen
Journal:  Infect Immun       Date:  1984-02       Impact factor: 3.441

5.  Nucleotide sequence of the gene for heat-stable enterotoxin II of Escherichia coli.

Authors:  R N Picken; A J Mazaitis; W K Maas; M Rey; H Heyneker
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441

6.  Production of K99 antigen by enterotoxigenic Escherichia coli strains of antigen groups o8, o9, o20, and o101 grown at different conditions.

Authors:  F K de Graaf; F B Wientjes; P Klaasen-Boor
Journal:  Infect Immun       Date:  1980-01       Impact factor: 3.441

7.  Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with entertoxigenicity.

Authors:  P A Guinée; W H Jansen; C M Agterberg
Journal:  Infect Immun       Date:  1976-05       Impact factor: 3.441

8.  Cloning, mapping and expression of the genetic determinant that encodes for the K88ab antigen.

Authors:  F R Mooi; F K de Graaf; J D van Embden
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

9.  Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin determinant.

Authors:  M So; W S Dallas; S Falkow
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

10.  Identification of enterotoxigenic Escherichia coli isolated from swine with diarrhea in Thailand by colony hybridization, using three enterotoxin gene probes.

Authors:  U Patamaroj; J Seriwatana; P Echeverria
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948
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  4 in total

1.  Use of nucleic acid probes in the diagnosis of diarrheal disorders.

Authors:  P Echeverria; D N Taylor; J Seriwatana; O Sethabutr; A Chatkaeomorakot
Journal:  Indian J Pediatr       Date:  1987 Jan-Feb       Impact factor: 1.967

2.  DNA-based diagnostic tests for Salmonella species targeting agfA, the structural gene for thin, aggregative fimbriae.

Authors:  J L Doran; S K Collinson; J Burian; G Sarlós; E C Todd; C K Munro; C M Kay; P A Banser; P I Peterkin; W W Kay
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

3.  Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis.

Authors:  C Sotiropoulos; P J Coloe; S C Smith
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948

4.  Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea.

Authors:  J Harel; H Lapointe; A Fallara; L A Lortie; M Bigras-Poulin; S Larivière; J M Fairbrother
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948
  4 in total

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