| Literature DB >> 2412940 |
S Person, S C Warner, D J Bzik, C Debroy, B A Fox.
Abstract
A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZ alpha expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].Entities:
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Year: 1985 PMID： 2412940 DOI： 10.1016/0378-1119(85)90006-x
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688