Potentiation of tachykinin-induced plasma protein extravasation by calcitonin gene-related peptide.

The effect of neuropeptides on plasma protein extravasation was investigated in the abdominal skin of rats. Substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) induced extravasation with a threshold dose of about 1 pmol. Calcitonin gene-related peptide (CGRP) was ineffective up to 6 pmol. However, when CGRP was injected together with either of the tachykinins extravasation was potentiated. A dose of 6 pmol CGRP shifted the dose-response curve of SP to the left by a factor of about 100. The vasoconstrictor neuropeptide Y (NPY, 12 pmol) reduced the extravasation caused by SP or SP plus 6 pmol CGRP. These results indicate that all 3 tachykinins currently known to be present in sensory neurons induce plasma protein extravasation, i.e. mimic one sign of neurogenic inflammation. This activity is potentiated in the presence of CGRP which coexists with SP and NKA indicating that neurogenic inflammation may be augmented by these interactions.