| Literature DB >> 24126086 |
Valeria F Del Coco1, María Alejandra Córdoba, Gladys Bilbao, Pinto de Almeida Castro, Juan Angel Basualdo, Mónica Santín.
Abstract
Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area.Entities:
Keywords: Argentina; Cattle; Enterocytozoon bieneusi; Genotype; Microsporidia; PCR
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Year: 2013 PMID： 24126086 DOI： 10.1016/j.vetpar.2013.09.024
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738