OBJECTIVES: To assess the feasibility of using a novel ultrasensitive bright-field in situ hybridization approach (BRISH) to evaluate κ and λ immunoglobulin messenger RNA (mRNA) expression in situ in B-cell non-Hodgkin lymphoma (NHL). METHODS: A series of 110 semiconsecutive clinical cases evaluated for lymphoma with historic flow cytometric (FCM) results were assessed with BRISH. RESULTS: BRISH light chain restriction (LCR) results were concordant with FCM in 108 (99%) of 109 evaluable cases. Additional small B-cell lymphoma cohorts were successfully evaluated. CONCLUSIONS: BRISH analysis of κ and λ immunoglobulin mRNA expression is a sensitive tool for establishing LCR in B-cell NHL when FCM results are not available.
OBJECTIVES: To assess the feasibility of using a novel ultrasensitive bright-field in situ hybridization approach (BRISH) to evaluate κ and λ immunoglobulin messenger RNA (mRNA) expression in situ in B-cell non-Hodgkin lymphoma (NHL). METHODS: A series of 110 semiconsecutive clinical cases evaluated for lymphoma with historic flow cytometric (FCM) results were assessed with BRISH. RESULTS: BRISH light chain restriction (LCR) results were concordant with FCM in 108 (99%) of 109 evaluable cases. Additional small B-cell lymphoma cohorts were successfully evaluated. CONCLUSIONS: BRISH analysis of κ and λ immunoglobulin mRNA expression is a sensitive tool for establishing LCR in B-cell NHL when FCM results are not available.
Authors: Kshitij Arora; Ivan Chebib; Lawrence Zukerberg; Manoj Gandhi; Miguel Rivera; David Ting; Vikram Deshpande Journal: Cancer Cytopathol Date: 2015-11-02 Impact factor: 5.284
Authors: Lisa M Rimsza; William A Day; Sarah McGinn; Anne Pedata; Yasodha Natkunam; Roger Warnke; James R Cook; Teresa Marafioti; Thomas M Grogan Journal: Diagn Pathol Date: 2014-07-21 Impact factor: 2.644
Authors: Ling Guo; Zhen Wang; Courtney M Anderson; Emerald Doolittle; Siobhan Kernag; Claudiu V Cotta; Sarah L Ondrejka; Xiao-Jun Ma; James R Cook Journal: Mod Pathol Date: 2017-10-20 Impact factor: 7.842