Xueyan Chen1, Sindhu Cherian. 1. Dept of Laboratory Medicine, University of Washington, 1959 NE Pacific St, Seattle, WA 98195; xchen1@u.washington.edu.
Abstract
OBJECTIVES: To review clinical data, cytogenetic findings, and flow cytometric analysis in 20 patients with T-cell prolymphocytic leukemia (T-PLL), a rare, aggressive, mature T-cell leukemia with poor prognosis and short survival. METHODS: Using multiparameter flow cytometry with a large combination of antibodies, we summarize the immunophenotypic features of T-PLL, including unusual immunophenotypic variants, and illustrate immunophenotypic clues that may help distinguish this entity from other T-cell malignancies. RESULTS: By flow cytometry, T-PLL is characterized by a postthymic mature T-cell immunophenotype with a variety of abnormalities that usually allow distinction from other mature T-cell leukemias. CONCLUSIONS: Although definitive diagnosis of T-PLL requires a systemic approach with integration of clinical data, morphology, immunophenotype, cytogenetics/fluorescence in situ hybridization, and molecular features, our results indicate immunophenotyping by multiparameter flow cytometry greatly facilitates diagnosis and assists with subclassification of this mature T-cell leukemia.
OBJECTIVES: To review clinical data, cytogenetic findings, and flow cytometric analysis in 20 patients with T-cell prolymphocytic leukemia (T-PLL), a rare, aggressive, mature T-cell leukemia with poor prognosis and short survival. METHODS: Using multiparameter flow cytometry with a large combination of antibodies, we summarize the immunophenotypic features of T-PLL, including unusual immunophenotypic variants, and illustrate immunophenotypic clues that may help distinguish this entity from other T-cell malignancies. RESULTS: By flow cytometry, T-PLL is characterized by a postthymic mature T-cell immunophenotype with a variety of abnormalities that usually allow distinction from other mature T-cell leukemias. CONCLUSIONS: Although definitive diagnosis of T-PLL requires a systemic approach with integration of clinical data, morphology, immunophenotype, cytogenetics/fluorescence in situ hybridization, and molecular features, our results indicate immunophenotyping by multiparameter flow cytometry greatly facilitates diagnosis and assists with subclassification of this mature T-cell leukemia.
Authors: Natali Pflug; Annika Littauer; David Beverungen; Aleksandra Sretenovic; Linus Wahnschaffe; Till Braun; Annika Dechow; Dennis Jungherz; Moritz Otte; Astrid Monecke; Enrica Bach; Georg-Nikolaus Franke; Sebastian Schwind; Madlen Jentzsch; Uwe Platzbecker; Marco Herling; Vladan Vucinic Journal: Front Oncol Date: 2022-01-05 Impact factor: 6.244
Authors: P Jain; E Aoki; M Keating; W G Wierda; S O'Brien; G N Gonzalez; A Ferrajoli; N Jain; P A Thompson; E Jabbour; R Kanagal-Shamanna; S Pierce; A Alousi; C Hosing; I Khouri; Z Estrov; J Cortes; H Kantarjian; F Ravandi; T M Kadia Journal: Ann Oncol Date: 2017-07-01 Impact factor: 32.976