OBJECTIVES: To determine whether the fraction of CD177+ neutrophils might be altered in clonal myeloid disorders, similar to the skewed κ/λ ratio for B-cell lymphomas, and could be used to identify myeloid neoplasms. METHODS: Blood and bone marrow samples were evaluated for the fraction of CD177+ neutrophils by flow cytometry. RESULTS: Skewed high neutrophil CD177(%) was not associated with neoplasia, but skewed low neutrophil CD177(%) was highly correlated with clonal myeloid disorders at values less than 40%. Specificity of low neutrophil CD177(%) for clonal myeloid disorders was 87% with a 40% cutoff and 95% with a 30% cutoff. Findings were most pronounced for myelodysplasia, with 52% (11/21) containing fewer than 40% CD177+ neutrophils. Specificity was also suggested by normalization of neutrophil CD177(%) in four patients who reached morphologic remission after therapy for myelodysplasia or acute leukemia. CONCLUSIONS: Skewed low neutrophil CD177(%) is highly associated with clonal myeloid disorders, particularly myelodysplasia, and may be useful for detecting clonal myeloid disorders.
OBJECTIVES: To determine whether the fraction of CD177+ neutrophils might be altered in clonal myeloid disorders, similar to the skewed κ/λ ratio for B-cell lymphomas, and could be used to identify myeloid neoplasms. METHODS: Blood and bone marrow samples were evaluated for the fraction of CD177+ neutrophils by flow cytometry. RESULTS: Skewed high neutrophil CD177(%) was not associated with neoplasia, but skewed low neutrophil CD177(%) was highly correlated with clonal myeloid disorders at values less than 40%. Specificity of low neutrophil CD177(%) for clonal myeloid disorders was 87% with a 40% cutoff and 95% with a 30% cutoff. Findings were most pronounced for myelodysplasia, with 52% (11/21) containing fewer than 40% CD177+ neutrophils. Specificity was also suggested by normalization of neutrophil CD177(%) in four patients who reached morphologic remission after therapy for myelodysplasia or acute leukemia. CONCLUSIONS: Skewed low neutrophil CD177(%) is highly associated with clonal myeloid disorders, particularly myelodysplasia, and may be useful for detecting clonal myeloid disorders.
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Authors: Claudia Eulenberg-Gustavus; Sylvia Bähring; Philipp G Maass; Friedrich C Luft; Ralph Kettritz Journal: J Exp Med Date: 2017-05-30 Impact factor: 14.307
Authors: Ana Aires; Maria Dos Anjos Teixeira; Catarina Lau; Cláudia Moreira; Ana Spínola; Alexandra Mota; Inês Freitas; Jorge Coutinho; Margarida Lima Journal: BMC Hematol Date: 2018-03-13
Authors: Zuopeng Wu; Rong Liang; Thomas Ohnesorg; Vicky Cho; Wesley Lam; Walter P Abhayaratna; Paul A Gatenby; Chandima Perera; Yafei Zhang; Belinda Whittle; Andrew Sinclair; Christopher C Goodnow; Matthew Field; T Daniel Andrews; Matthew C Cook Journal: PLoS Genet Date: 2016-05-26 Impact factor: 5.917