BACKGROUND/AIM: The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). MATERIALS AND METHODS: Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. RESULTS: LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. CONCLUSION: EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.
BACKGROUND/AIM: The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a humanoesophageal carcinoma cell line (TE-1). MATERIALS AND METHODS:Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. RESULTS: LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. CONCLUSION:EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.
Authors: Domingo J Ramos-Campo; Vicente Ávila-Gandía; Fco Javier López-Román; José Miñarro; Carlos Contreras; Fulgencio Soto-Méndez; Joan C Domingo Pedrol; Antonio J Luque-Rubia Journal: Nutrients Date: 2020-03-09 Impact factor: 5.717
Authors: Vicente Ávila-Gandía; Antonio Torregrosa-García; Antonio J Luque-Rubia; María Salud Abellán-Ruiz; Desirée Victoria-Montesinos; F Javier López-Román Journal: J Int Soc Sports Nutr Date: 2020-10-21 Impact factor: 5.150
Authors: Alessandra Borsini; Anna Nicolaou; Dolores Camacho-Muñoz; Alexandra C Kendall; Maria Grazia Di Benedetto; Juliette Giacobbe; Kuan-Pin Su; Carmine M Pariante Journal: Mol Psychiatry Date: 2021-06-16 Impact factor: 15.992