The effect of Na+ and Cl- removal and of loop diuretics on acetylcholine-evoked membrane potential changes in mouse lacrimal acinar cells.

Intracellular micro-electrode recordings were made from acinar units in mouse lacrimal gland segments superfused with physiological saline solutions. Two micro-electrodes were used: one for recording the membrane potential and the other for current injection. A third extracellular micropipette was used for local ionophoretic acetylcholine (ACh) application. The ACh-evoked membrane potential change was recorded at the spontaneous resting potential and after the resting potential had been increased or decreased by direct current injection. The membrane potential at which ACh did not evoke any change in potential (null potential, reversal potential) (EACh) was determined. EACh was about -53 mV under normal ionic conditions. When all Cl- in the superfusion fluid was replaced by NO3- or all Na+ replaced by N-methyl-D-glucamine+ (NMDG+) or Tris+, EACh was shifted to more negative values. During exposure to Cl(-)-free NO3- solution or Na+-free NMDG+ solution EACh was about -72 mV. These effects on EACh were fully reversible. Inclusion of the loop diuretics piretanide (2 x 10(-4) M) or furosemide (10(-3) M) also shifted EACh towards more negative values. In these cases EACh was the same as during exposure to Na+-free Tris+ solution with a value of about -62 mV. Replacement of extracellular Cl- by Br- had no effect on EACh whereas replacement of Na+ by Li+ shifted Each towards less negative values.(ABSTRACT TRUNCATED AT 250 WORDS)