| Literature DB >> 24121002 |
Yadong Yang1, Hai Wang1, Kai-Hsin Chang2, Hongzhu Qu1, Zhaojun Zhang1, Qian Xiong1, Heyuan Qi1, Peng Cui3, Qiang Lin3, Xiuyan Ruan1, Yaran Yang1, Yajuan Li1, Chang Shu3, Quanzhen Li4, Edward K Wakeland1,4, Jiangwei Yan1, Songnian Hu3, Xiangdong Fang1.
Abstract
To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.Entities:
Keywords: Cell differentiation; Development; Erythropoiesis; Gene regulatory networks; High-throughput RNA sequencing
Mesh：
Year: 2013 PMID： 24121002 PMCID： PMC4151266 DOI： 10.1016/j.ygeno.2013.09.005
Source DB: PubMed Journal: Genomics ISSN： 0888-7543 Impact factor: 5.736